Cytotoxic Activity and Antibiofilm Efficacy of Biosynthesized Silver Nanoparticles against Methicillin-Resistant Staphylococcus aureus Strains Colonizing Cell Phones

The interest for green synthesis of metallic nanoparticles (NPs) has acquired particular attention due to its low toxicity and economic feasibility compared with chemical or physical process. Here we carried out an extracellular synthesis approach of silver nanoparticles (AgNPs) using dried orange p...

Full description

Saved in:
Bibliographic Details
Published in:The Canadian journal of infectious diseases & medical microbiology 2022-03, Vol.2022, p.9410024-10
Main Authors: Merghni, Abderrahmen, Lassoued, Mohamed Ali, Noumi, Emira, Hadj Lajimi, Ramzi, Adnan, Mohd, Mastouri, Maha, Snoussi, Mejdi
Format: Article
Language:English
Subjects:
Anti-infective agents
Antibiotics
Anticancer properties
Antimicrobial agents
Antitumor activity
Aqueous solutions
Bacteria
Biofilms
Biological properties
Biosynthesis
Cell phones
Cellular telephones
Chemical properties
Chemical research
Citrus
Citrus fruits
Contamination
Control
Cytotoxicity
Drug resistance
Drug resistance in microorganisms
Electron microscopes
Enzymes
Fourier transforms
Functional groups
Infectious diseases
Infrared analysis
Infrared spectroscopy
Light scattering
Materia medica, Vegetable
Melanoma
Methicillin
Microorganisms
Microscopes
Minimum inhibitory concentration
Nanoparticles
Orange oil
Particle size
Photon correlation spectroscopy
Plant extracts
Silver
Spectrum analysis
Stability analysis
Staphylococcus aureus
Staphylococcus aureus infections
Staphylococcus infections
Strains (organisms)
Toxicity
Transmission electron microscopes
Transmission electron microscopy
Virulence
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The interest for green synthesis of metallic nanoparticles (NPs) has acquired particular attention due to its low toxicity and economic feasibility compared with chemical or physical process. Here we carried out an extracellular synthesis approach of silver nanoparticles (AgNPs) using dried orange peel extract. Characterization studies revealed the synthesis of 25–30 nm AgNPs with distinct morphology as observed in transmission electron microscopes. Dynamic light scattering spectroscopy and Fourier transform infrared spectroscopy analyses further characterized nanoparticles confirming their stability and the presence of functional groups. The biological properties of biosynthesized AgNPs were subsequently investigated. Our results revealed anticancer activity of biogenic silver NPs against the B16 melanoma cell line with an IC50 value of 25 µg/ml. Additionally, the developed AgNPs displayed a considerable antagonistic activity against methicillin-resistant Staphylococcus aureus (MRSA) strains colonizing cell phones, with inhibition zones between 12 and 14 mm and minimum inhibitory concentration values between 1.56 and 12.5 µg/ml. Furthermore, the AgNPs exhibited potent antibiofilm activity against MRSA strains with the percent biofilm disruption reaching 80%. Our results highlighted the efficacy of biosynthesized AgNPs against bacterial biofilms and pointed to the exploration of orange peels as a natural and cost-effective strategy.
ISSN:1712-9532
1918-1493
DOI:10.1155/2022/9410024