Enhanced vacuole compartmentalization of cadmium in root cells contributes to glutathione-induced reduction of cadmium translocation from roots to shoots in pakchoi (Brassica chinensis L.)

• Published in: Ecotoxicology and environmental safety 2021-01, Vol.208, p.111616, Article 111616
• Main Authors: Huang, Yifan, Chen, Jiahui, Zhang, Derui, Fang, Bo, YangJin, Tsering, Zou, Jianwen, Chen, Yahua, Su, Nana, Cui, Jin
• Format: Article
• Language: English
• Subjects: Biological Transport; Brassica - drug effects; Brassica - genetics; Brassica - metabolism; Cadmium; Cadmium - metabolism; Gene Expression Regulation, Plant - drug effects; Glutathione; Glutathione - metabolism; Pakchoi; Phytochelatins; Plant Proteins - genetics; Plant Roots - metabolism; Seedlings - drug effects; Seedlings - metabolism; Soil Pollutants - metabolism; Vacuole compartmentalization; Vacuoles - metabolism
• ISSN: 0147-6513; 1090-2414
• DOI: 10.1016/j.ecoenv.2020.111616

Our previous studies showed that exogenous glutathione (GSH) decreased cadmium (Cd) concentration in shoots and alleviated the growth inhibition in pakchoi (Brassica chinensis L.) under Cd stress. Nevertheless, it is largely unknown how GSH decreases Cd accumulation in edible parts of pakchoi. This experiment mainly explored the mechanisms of GSH-induced reduction of Cd accumulation in shoot of pakchoi. The results showed that compared with sole Cd treatment, Cd + GSH treatment remarkably increased the expression of BcIRT1 and BcIRT2, and further enhanced the concentrations of Cd and Fe in root. By contrast, GSH application declined the concentration of Cd in the xylem sap. However, these results were not caused by xylem loading process because the expression of BcHMA2 and BcHMA4 had not significant difference between sole Cd treatment and Cd + GSH treatment. In addition, exogenous GSH significantly enhanced the expression of BcPCS1 and promoted the synthesis of PC2, PC3 and PC4 under Cd stress. At the same time, exogenous GSH also significantly improved the expression of BcABCC1 and BcABCC2 in the roots of seedling under Cd stress, suggesting that more PCs-Cd complexes may be sequestrated into vacuoles by ABCC1 and ABCC2 transporters. The results showed that exogenous GSH could up-regulate the expression of BcIRT1/2 to increase the Cd accumulation in root, and the improvement of PCs contents and the expression of BcABCC1/2 enhanced the compartmentalization of Cd in root vacuole of pakchoi under Cd stress. To sum up, exogenous GSH reduce the concentration of free Cd2+ in the cytoplast of root cells and then dropped the loading of Cd into the xylem, which eventually given rise to the reduction of Cd accumulation in edible portion of pakchoi. •Exogenous GSH can remarkably inhibit Cd translocation from root to shoot in pakchoi when exposed to Cd stress.•Exogenous GSH could promote the absorption of Cd and Fe by inducing BcIRT1 and BcIRT1 gene expression in root.•Exogenous GSH promote the form of PCs-Cd complexes which are transported into root vacuole through BcABCC1 and BcABCC2.