Comparative Analysis of Tissue Copper Levels in Oral Submucous Fibrosis (OSMF) Patients

Oral submucous fibrosis (OSMF) is a recognized potentially malignant oral condition linked to the consumption of areca nut. Chewing areca nut has been shown to elevate soluble copper levels in mouth fluids. Participants: The study included a panel of 30 patients with OSMF from Rama Dental College, K...

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Published in:Journal of pharmacy & bioallied science 2024-02, Vol.16 (Suppl 1), p.S629-S630
Main Authors: Srivastava, Rahul, Singh, Dhirendra K, Thomas, Pallavi A, Raza, Hadi, Sangamesh, N C, Bagde, Hiroj S
Format: Article
Language:English
Subjects:
Analysis
Areca catechu
areca nut chewing
Biopsy
Chewing
Comparative analysis
Connective tissues
Copper
Epithelium
Fibrosis
lysyl oxidase activity
Mass spectroscopy
Medical research
Medicine, Experimental
Mucosa
Nut industry
Nuts
Oral fluids
oral submucous fibrosis
Original
Scientific imaging
serum copper levels
Statistical analysis
Statistics
tissue copper concentrations
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Summary:Oral submucous fibrosis (OSMF) is a recognized potentially malignant oral condition linked to the consumption of areca nut. Chewing areca nut has been shown to elevate soluble copper levels in mouth fluids. Participants: The study included a panel of 30 patients with OSMF from Rama Dental College, Kanpur, India, and 30 nonareca chewing individuals serving as controls. Tissue Sample Collection and Analysis: Buccal mucosal biopsies were obtained from both OSMF patients and controls. The tissue copper concentrations were quantified using mass absorption spectrometry (MAS). Additionally, energy-dispersive X-ray microanalysis (EDX) was employed to identify the presence and distribution of copper in the tissue. Statistical comparisons were performed using appropriate methods, with a value of less than 0.05 considered statistically significant. MAS analysis revealed that the mean tissue copper level was 6.2 ± 3.1 micrograms per gram (μg/g) in OSMF specimens ( = 30), slightly higher than the 4.5 ± 2.0 μg/g in the nonareca chewing controls ( = 30) ( = 0.1). EDX analysis showed distinct copper peaks in both the epithelium (22/23) and connective tissue (18/23) of OSMF specimens compared to control biopsies. These findings were corroborated by secondary ion mass spectrometry (SIMS) in a subset of samples. The study revealed higher copper concentrations in buccal mucosal tissue of OSMF patients from Rama Dental College, Kanpur, suggesting a potential connection between copper and the initiation of OSMF.
ISSN:0976-4879
0975-7406
DOI:10.4103/jpbs.jpbs_904_23