Loading…

Species-Specific Analysis of Bacterial Vaginosis-Associated Bacteria

Vaginal dysbiosis in women reduces the abundance of species and increases that of anaerobic fastidious bacteria. This dysbiotic condition in the vagina, called bacterial vaginosis (BV), can be symptomatic with odorous vaginal discharges or asymptomatic and affects a third of women of reproductive ag...

Full description

Saved in:
Bibliographic Details
Published in:Microbiology spectrum 2023-08, Vol.11 (4), p.e0467622-e0467622
Main Authors: Osei Sekyere, John, Oyenihi, Ayodeji B, Trama, Jason, Adelson, Martin E
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c536t-62da05b0a21b36412e045b865e7925f840586dcec50094b96a04d1566bd49a883
cites cdi_FETCH-LOGICAL-c536t-62da05b0a21b36412e045b865e7925f840586dcec50094b96a04d1566bd49a883
container_end_page e0467622
container_issue 4
container_start_page e0467622
container_title Microbiology spectrum
container_volume 11
creator Osei Sekyere, John
Oyenihi, Ayodeji B
Trama, Jason
Adelson, Martin E
description Vaginal dysbiosis in women reduces the abundance of species and increases that of anaerobic fastidious bacteria. This dysbiotic condition in the vagina, called bacterial vaginosis (BV), can be symptomatic with odorous vaginal discharges or asymptomatic and affects a third of women of reproductive age. Three unclassified bacterial species designated BV-associated bacteria 1, 2, and 3 (BVAB-1, -2, and -3) in 2005 were found to be highly preponderant in the vagina of females with BV. Here, we used sequence homology and phylogenetics analyses to identify the actual species of BVAB-1, -2, and -3 and found BVAB-1 to be genomosp. BVAB-1, BVAB-2 to be bacterium strain CHIC02, and BVAB-3 to be Mageeibacillus indolicus, respectively. These are anaerobic and uncultured species that can be identified only through metagenomics. Long-read sequencing of BV specimens can also enable a genomic reassembly of these species' genomes from metagenomes. Species-specific identification of these pathogens and the availability of their genomes from assembled metagenomes will advance our understanding of their biology, facilitate the design of sensitive diagnostics and drugs, and enhance the treatment of BV. For many years since 2005, BVAB, an important pathogen of the female vaginal tract that is associated with BV, has been identified using PCR without knowing its actual species. Without a full genome of these pathogens, a better understanding of their pathogenicity, treatment, resistance, and diagnostics cannot be reached. In this analysis, we use the DNA of BVAB-1, -2, and -3 to determine their actual species to enhance further research into their pathogenicity, resistance, diagnosis, and treatment.
doi_str_mv 10.1128/spectrum.04676-22
format article
fullrecord <record><control><sourceid>proquest_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_a9163b59183d49098a0bb56772bd580b</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_a9163b59183d49098a0bb56772bd580b</doaj_id><sourcerecordid>2828772577</sourcerecordid><originalsourceid>FETCH-LOGICAL-c536t-62da05b0a21b36412e045b865e7925f840586dcec50094b96a04d1566bd49a883</originalsourceid><addsrcrecordid>eNpVkU9PHDEMxaOqVUGUD9BLtcdehjrO3zlVWwoUCakHaK-Rk8lsg2Yn22S2Et-e6S6s4GTL9vs9S4-xjxzOOEf7pW5imMp2fQZSG90gvmHHyLVqQLbm7Yv-iJ3Weg8AnINChe_ZkTBCGgQ8Zt9vZ0yKtdnVPoXFcqThoaa6yP3iG4UplkTD4jet0pjncbOsNYdEU-wO6w_sXU9DjadP9YT9ury4O__R3Py8uj5f3jRBCT01GjsC5YGQe6ElxwhSeatVNC2q3kpQVnchBgXQSt9qAtlxpbXvZEvWihN2ved2me7dpqQ1lQeXKbndIJeVozKlMERHLdfCq5ZbMYuhtQTeK20M-k5Z8DPr65612fp1nF3HqdDwCvp6M6Y_bpX_OQ5SSG7MTPj8RCj57zbWya1TDXEYaIx5Wx1atLOf2p3y_WkoudYS-4MPB_c_Tfecptul6RBnzaeXDx4Uz9mJR4u7m_Q</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2828772577</pqid></control><display><type>article</type><title>Species-Specific Analysis of Bacterial Vaginosis-Associated Bacteria</title><source>PubMed Central (PMC)</source><source>American Society for Microbiology Journals</source><creator>Osei Sekyere, John ; Oyenihi, Ayodeji B ; Trama, Jason ; Adelson, Martin E</creator><contributor>Frese, Steven</contributor><creatorcontrib>Osei Sekyere, John ; Oyenihi, Ayodeji B ; Trama, Jason ; Adelson, Martin E ; Frese, Steven</creatorcontrib><description>Vaginal dysbiosis in women reduces the abundance of species and increases that of anaerobic fastidious bacteria. This dysbiotic condition in the vagina, called bacterial vaginosis (BV), can be symptomatic with odorous vaginal discharges or asymptomatic and affects a third of women of reproductive age. Three unclassified bacterial species designated BV-associated bacteria 1, 2, and 3 (BVAB-1, -2, and -3) in 2005 were found to be highly preponderant in the vagina of females with BV. Here, we used sequence homology and phylogenetics analyses to identify the actual species of BVAB-1, -2, and -3 and found BVAB-1 to be genomosp. BVAB-1, BVAB-2 to be bacterium strain CHIC02, and BVAB-3 to be Mageeibacillus indolicus, respectively. These are anaerobic and uncultured species that can be identified only through metagenomics. Long-read sequencing of BV specimens can also enable a genomic reassembly of these species' genomes from metagenomes. Species-specific identification of these pathogens and the availability of their genomes from assembled metagenomes will advance our understanding of their biology, facilitate the design of sensitive diagnostics and drugs, and enhance the treatment of BV. For many years since 2005, BVAB, an important pathogen of the female vaginal tract that is associated with BV, has been identified using PCR without knowing its actual species. Without a full genome of these pathogens, a better understanding of their pathogenicity, treatment, resistance, and diagnostics cannot be reached. In this analysis, we use the DNA of BVAB-1, -2, and -3 to determine their actual species to enhance further research into their pathogenicity, resistance, diagnosis, and treatment.</description><identifier>ISSN: 2165-0497</identifier><identifier>EISSN: 2165-0497</identifier><identifier>DOI: 10.1128/spectrum.04676-22</identifier><identifier>PMID: 37347202</identifier><language>eng</language><publisher>United States: American Society for Microbiology</publisher><subject>BVAB ; metagenome-assembled genome ; Observation ; vaginal microbiome</subject><ispartof>Microbiology spectrum, 2023-08, Vol.11 (4), p.e0467622-e0467622</ispartof><rights>Copyright © 2023 Osei Sekyere et al. 2023 Osei Sekyere et al.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c536t-62da05b0a21b36412e045b865e7925f840586dcec50094b96a04d1566bd49a883</citedby><cites>FETCH-LOGICAL-c536t-62da05b0a21b36412e045b865e7925f840586dcec50094b96a04d1566bd49a883</cites><orcidid>0000-0002-9508-984X ; 0000-0001-9912-1539 ; 0000-0002-1147-6325</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10434177/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10434177/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,3188,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37347202$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Frese, Steven</contributor><creatorcontrib>Osei Sekyere, John</creatorcontrib><creatorcontrib>Oyenihi, Ayodeji B</creatorcontrib><creatorcontrib>Trama, Jason</creatorcontrib><creatorcontrib>Adelson, Martin E</creatorcontrib><title>Species-Specific Analysis of Bacterial Vaginosis-Associated Bacteria</title><title>Microbiology spectrum</title><addtitle>Microbiol Spectr</addtitle><description>Vaginal dysbiosis in women reduces the abundance of species and increases that of anaerobic fastidious bacteria. This dysbiotic condition in the vagina, called bacterial vaginosis (BV), can be symptomatic with odorous vaginal discharges or asymptomatic and affects a third of women of reproductive age. Three unclassified bacterial species designated BV-associated bacteria 1, 2, and 3 (BVAB-1, -2, and -3) in 2005 were found to be highly preponderant in the vagina of females with BV. Here, we used sequence homology and phylogenetics analyses to identify the actual species of BVAB-1, -2, and -3 and found BVAB-1 to be genomosp. BVAB-1, BVAB-2 to be bacterium strain CHIC02, and BVAB-3 to be Mageeibacillus indolicus, respectively. These are anaerobic and uncultured species that can be identified only through metagenomics. Long-read sequencing of BV specimens can also enable a genomic reassembly of these species' genomes from metagenomes. Species-specific identification of these pathogens and the availability of their genomes from assembled metagenomes will advance our understanding of their biology, facilitate the design of sensitive diagnostics and drugs, and enhance the treatment of BV. For many years since 2005, BVAB, an important pathogen of the female vaginal tract that is associated with BV, has been identified using PCR without knowing its actual species. Without a full genome of these pathogens, a better understanding of their pathogenicity, treatment, resistance, and diagnostics cannot be reached. In this analysis, we use the DNA of BVAB-1, -2, and -3 to determine their actual species to enhance further research into their pathogenicity, resistance, diagnosis, and treatment.</description><subject>BVAB</subject><subject>metagenome-assembled genome</subject><subject>Observation</subject><subject>vaginal microbiome</subject><issn>2165-0497</issn><issn>2165-0497</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNpVkU9PHDEMxaOqVUGUD9BLtcdehjrO3zlVWwoUCakHaK-Rk8lsg2Yn22S2Et-e6S6s4GTL9vs9S4-xjxzOOEf7pW5imMp2fQZSG90gvmHHyLVqQLbm7Yv-iJ3Weg8AnINChe_ZkTBCGgQ8Zt9vZ0yKtdnVPoXFcqThoaa6yP3iG4UplkTD4jet0pjncbOsNYdEU-wO6w_sXU9DjadP9YT9ury4O__R3Py8uj5f3jRBCT01GjsC5YGQe6ElxwhSeatVNC2q3kpQVnchBgXQSt9qAtlxpbXvZEvWihN2ved2me7dpqQ1lQeXKbndIJeVozKlMERHLdfCq5ZbMYuhtQTeK20M-k5Z8DPr65612fp1nF3HqdDwCvp6M6Y_bpX_OQ5SSG7MTPj8RCj57zbWya1TDXEYaIx5Wx1atLOf2p3y_WkoudYS-4MPB_c_Tfecptul6RBnzaeXDx4Uz9mJR4u7m_Q</recordid><startdate>20230817</startdate><enddate>20230817</enddate><creator>Osei Sekyere, John</creator><creator>Oyenihi, Ayodeji B</creator><creator>Trama, Jason</creator><creator>Adelson, Martin E</creator><general>American Society for Microbiology</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-9508-984X</orcidid><orcidid>https://orcid.org/0000-0001-9912-1539</orcidid><orcidid>https://orcid.org/0000-0002-1147-6325</orcidid></search><sort><creationdate>20230817</creationdate><title>Species-Specific Analysis of Bacterial Vaginosis-Associated Bacteria</title><author>Osei Sekyere, John ; Oyenihi, Ayodeji B ; Trama, Jason ; Adelson, Martin E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c536t-62da05b0a21b36412e045b865e7925f840586dcec50094b96a04d1566bd49a883</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>BVAB</topic><topic>metagenome-assembled genome</topic><topic>Observation</topic><topic>vaginal microbiome</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Osei Sekyere, John</creatorcontrib><creatorcontrib>Oyenihi, Ayodeji B</creatorcontrib><creatorcontrib>Trama, Jason</creatorcontrib><creatorcontrib>Adelson, Martin E</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals (DOAJ)</collection><jtitle>Microbiology spectrum</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Osei Sekyere, John</au><au>Oyenihi, Ayodeji B</au><au>Trama, Jason</au><au>Adelson, Martin E</au><au>Frese, Steven</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Species-Specific Analysis of Bacterial Vaginosis-Associated Bacteria</atitle><jtitle>Microbiology spectrum</jtitle><addtitle>Microbiol Spectr</addtitle><date>2023-08-17</date><risdate>2023</risdate><volume>11</volume><issue>4</issue><spage>e0467622</spage><epage>e0467622</epage><pages>e0467622-e0467622</pages><issn>2165-0497</issn><eissn>2165-0497</eissn><abstract>Vaginal dysbiosis in women reduces the abundance of species and increases that of anaerobic fastidious bacteria. This dysbiotic condition in the vagina, called bacterial vaginosis (BV), can be symptomatic with odorous vaginal discharges or asymptomatic and affects a third of women of reproductive age. Three unclassified bacterial species designated BV-associated bacteria 1, 2, and 3 (BVAB-1, -2, and -3) in 2005 were found to be highly preponderant in the vagina of females with BV. Here, we used sequence homology and phylogenetics analyses to identify the actual species of BVAB-1, -2, and -3 and found BVAB-1 to be genomosp. BVAB-1, BVAB-2 to be bacterium strain CHIC02, and BVAB-3 to be Mageeibacillus indolicus, respectively. These are anaerobic and uncultured species that can be identified only through metagenomics. Long-read sequencing of BV specimens can also enable a genomic reassembly of these species' genomes from metagenomes. Species-specific identification of these pathogens and the availability of their genomes from assembled metagenomes will advance our understanding of their biology, facilitate the design of sensitive diagnostics and drugs, and enhance the treatment of BV. For many years since 2005, BVAB, an important pathogen of the female vaginal tract that is associated with BV, has been identified using PCR without knowing its actual species. Without a full genome of these pathogens, a better understanding of their pathogenicity, treatment, resistance, and diagnostics cannot be reached. In this analysis, we use the DNA of BVAB-1, -2, and -3 to determine their actual species to enhance further research into their pathogenicity, resistance, diagnosis, and treatment.</abstract><cop>United States</cop><pub>American Society for Microbiology</pub><pmid>37347202</pmid><doi>10.1128/spectrum.04676-22</doi><orcidid>https://orcid.org/0000-0002-9508-984X</orcidid><orcidid>https://orcid.org/0000-0001-9912-1539</orcidid><orcidid>https://orcid.org/0000-0002-1147-6325</orcidid><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 2165-0497
ispartof Microbiology spectrum, 2023-08, Vol.11 (4), p.e0467622-e0467622
issn 2165-0497
2165-0497
language eng
recordid cdi_doaj_primary_oai_doaj_org_article_a9163b59183d49098a0bb56772bd580b
source PubMed Central (PMC); American Society for Microbiology Journals
subjects BVAB
metagenome-assembled genome
Observation
vaginal microbiome
title Species-Specific Analysis of Bacterial Vaginosis-Associated Bacteria
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T08%3A06%3A14IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Species-Specific%20Analysis%20of%20Bacterial%20Vaginosis-Associated%20Bacteria&rft.jtitle=Microbiology%20spectrum&rft.au=Osei%20Sekyere,%20John&rft.date=2023-08-17&rft.volume=11&rft.issue=4&rft.spage=e0467622&rft.epage=e0467622&rft.pages=e0467622-e0467622&rft.issn=2165-0497&rft.eissn=2165-0497&rft_id=info:doi/10.1128/spectrum.04676-22&rft_dat=%3Cproquest_doaj_%3E2828772577%3C/proquest_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c536t-62da05b0a21b36412e045b865e7925f840586dcec50094b96a04d1566bd49a883%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2828772577&rft_id=info:pmid/37347202&rfr_iscdi=true