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Species-Specific Analysis of Bacterial Vaginosis-Associated Bacteria
Vaginal dysbiosis in women reduces the abundance of species and increases that of anaerobic fastidious bacteria. This dysbiotic condition in the vagina, called bacterial vaginosis (BV), can be symptomatic with odorous vaginal discharges or asymptomatic and affects a third of women of reproductive ag...
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Published in: | Microbiology spectrum 2023-08, Vol.11 (4), p.e0467622-e0467622 |
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description | Vaginal dysbiosis in women reduces the abundance of
species and increases that of anaerobic fastidious bacteria. This dysbiotic condition in the vagina, called bacterial vaginosis (BV), can be symptomatic with odorous vaginal discharges or asymptomatic and affects a third of women of reproductive age. Three unclassified bacterial species designated BV-associated bacteria 1, 2, and 3 (BVAB-1, -2, and -3) in 2005 were found to be highly preponderant in the vagina of females with BV. Here, we used sequence homology and phylogenetics analyses to identify the actual species of BVAB-1, -2, and -3 and found BVAB-1 to be
genomosp. BVAB-1, BVAB-2 to be
bacterium strain CHIC02, and BVAB-3 to be Mageeibacillus indolicus, respectively. These are anaerobic and uncultured species that can be identified only through metagenomics. Long-read sequencing of BV specimens can also enable a genomic reassembly of these species' genomes from metagenomes. Species-specific identification of these pathogens and the availability of their genomes from assembled metagenomes will advance our understanding of their biology, facilitate the design of sensitive diagnostics and drugs, and enhance the treatment of BV.
For many years since 2005, BVAB, an important pathogen of the female vaginal tract that is associated with BV, has been identified using PCR without knowing its actual species. Without a full genome of these pathogens, a better understanding of their pathogenicity, treatment, resistance, and diagnostics cannot be reached. In this analysis, we use the DNA of BVAB-1, -2, and -3 to determine their actual species to enhance further research into their pathogenicity, resistance, diagnosis, and treatment. |
doi_str_mv | 10.1128/spectrum.04676-22 |
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species and increases that of anaerobic fastidious bacteria. This dysbiotic condition in the vagina, called bacterial vaginosis (BV), can be symptomatic with odorous vaginal discharges or asymptomatic and affects a third of women of reproductive age. Three unclassified bacterial species designated BV-associated bacteria 1, 2, and 3 (BVAB-1, -2, and -3) in 2005 were found to be highly preponderant in the vagina of females with BV. Here, we used sequence homology and phylogenetics analyses to identify the actual species of BVAB-1, -2, and -3 and found BVAB-1 to be
genomosp. BVAB-1, BVAB-2 to be
bacterium strain CHIC02, and BVAB-3 to be Mageeibacillus indolicus, respectively. These are anaerobic and uncultured species that can be identified only through metagenomics. Long-read sequencing of BV specimens can also enable a genomic reassembly of these species' genomes from metagenomes. Species-specific identification of these pathogens and the availability of their genomes from assembled metagenomes will advance our understanding of their biology, facilitate the design of sensitive diagnostics and drugs, and enhance the treatment of BV.
For many years since 2005, BVAB, an important pathogen of the female vaginal tract that is associated with BV, has been identified using PCR without knowing its actual species. Without a full genome of these pathogens, a better understanding of their pathogenicity, treatment, resistance, and diagnostics cannot be reached. In this analysis, we use the DNA of BVAB-1, -2, and -3 to determine their actual species to enhance further research into their pathogenicity, resistance, diagnosis, and treatment.</description><identifier>ISSN: 2165-0497</identifier><identifier>EISSN: 2165-0497</identifier><identifier>DOI: 10.1128/spectrum.04676-22</identifier><identifier>PMID: 37347202</identifier><language>eng</language><publisher>United States: American Society for Microbiology</publisher><subject>BVAB ; metagenome-assembled genome ; Observation ; vaginal microbiome</subject><ispartof>Microbiology spectrum, 2023-08, Vol.11 (4), p.e0467622-e0467622</ispartof><rights>Copyright © 2023 Osei Sekyere et al. 2023 Osei Sekyere et al.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c536t-62da05b0a21b36412e045b865e7925f840586dcec50094b96a04d1566bd49a883</citedby><cites>FETCH-LOGICAL-c536t-62da05b0a21b36412e045b865e7925f840586dcec50094b96a04d1566bd49a883</cites><orcidid>0000-0002-9508-984X ; 0000-0001-9912-1539 ; 0000-0002-1147-6325</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10434177/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10434177/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,3188,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37347202$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Frese, Steven</contributor><creatorcontrib>Osei Sekyere, John</creatorcontrib><creatorcontrib>Oyenihi, Ayodeji B</creatorcontrib><creatorcontrib>Trama, Jason</creatorcontrib><creatorcontrib>Adelson, Martin E</creatorcontrib><title>Species-Specific Analysis of Bacterial Vaginosis-Associated Bacteria</title><title>Microbiology spectrum</title><addtitle>Microbiol Spectr</addtitle><description>Vaginal dysbiosis in women reduces the abundance of
species and increases that of anaerobic fastidious bacteria. This dysbiotic condition in the vagina, called bacterial vaginosis (BV), can be symptomatic with odorous vaginal discharges or asymptomatic and affects a third of women of reproductive age. Three unclassified bacterial species designated BV-associated bacteria 1, 2, and 3 (BVAB-1, -2, and -3) in 2005 were found to be highly preponderant in the vagina of females with BV. Here, we used sequence homology and phylogenetics analyses to identify the actual species of BVAB-1, -2, and -3 and found BVAB-1 to be
genomosp. BVAB-1, BVAB-2 to be
bacterium strain CHIC02, and BVAB-3 to be Mageeibacillus indolicus, respectively. These are anaerobic and uncultured species that can be identified only through metagenomics. Long-read sequencing of BV specimens can also enable a genomic reassembly of these species' genomes from metagenomes. Species-specific identification of these pathogens and the availability of their genomes from assembled metagenomes will advance our understanding of their biology, facilitate the design of sensitive diagnostics and drugs, and enhance the treatment of BV.
For many years since 2005, BVAB, an important pathogen of the female vaginal tract that is associated with BV, has been identified using PCR without knowing its actual species. Without a full genome of these pathogens, a better understanding of their pathogenicity, treatment, resistance, and diagnostics cannot be reached. In this analysis, we use the DNA of BVAB-1, -2, and -3 to determine their actual species to enhance further research into their pathogenicity, resistance, diagnosis, and treatment.</description><subject>BVAB</subject><subject>metagenome-assembled genome</subject><subject>Observation</subject><subject>vaginal microbiome</subject><issn>2165-0497</issn><issn>2165-0497</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNpVkU9PHDEMxaOqVUGUD9BLtcdehjrO3zlVWwoUCakHaK-Rk8lsg2Yn22S2Et-e6S6s4GTL9vs9S4-xjxzOOEf7pW5imMp2fQZSG90gvmHHyLVqQLbm7Yv-iJ3Weg8AnINChe_ZkTBCGgQ8Zt9vZ0yKtdnVPoXFcqThoaa6yP3iG4UplkTD4jet0pjncbOsNYdEU-wO6w_sXU9DjadP9YT9ury4O__R3Py8uj5f3jRBCT01GjsC5YGQe6ElxwhSeatVNC2q3kpQVnchBgXQSt9qAtlxpbXvZEvWihN2ved2me7dpqQ1lQeXKbndIJeVozKlMERHLdfCq5ZbMYuhtQTeK20M-k5Z8DPr65612fp1nF3HqdDwCvp6M6Y_bpX_OQ5SSG7MTPj8RCj57zbWya1TDXEYaIx5Wx1atLOf2p3y_WkoudYS-4MPB_c_Tfecptul6RBnzaeXDx4Uz9mJR4u7m_Q</recordid><startdate>20230817</startdate><enddate>20230817</enddate><creator>Osei Sekyere, John</creator><creator>Oyenihi, Ayodeji B</creator><creator>Trama, Jason</creator><creator>Adelson, Martin E</creator><general>American Society for Microbiology</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-9508-984X</orcidid><orcidid>https://orcid.org/0000-0001-9912-1539</orcidid><orcidid>https://orcid.org/0000-0002-1147-6325</orcidid></search><sort><creationdate>20230817</creationdate><title>Species-Specific Analysis of Bacterial Vaginosis-Associated Bacteria</title><author>Osei Sekyere, John ; Oyenihi, Ayodeji B ; Trama, Jason ; Adelson, Martin E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c536t-62da05b0a21b36412e045b865e7925f840586dcec50094b96a04d1566bd49a883</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>BVAB</topic><topic>metagenome-assembled genome</topic><topic>Observation</topic><topic>vaginal microbiome</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Osei Sekyere, John</creatorcontrib><creatorcontrib>Oyenihi, Ayodeji B</creatorcontrib><creatorcontrib>Trama, Jason</creatorcontrib><creatorcontrib>Adelson, Martin E</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals (DOAJ)</collection><jtitle>Microbiology spectrum</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Osei Sekyere, John</au><au>Oyenihi, Ayodeji B</au><au>Trama, Jason</au><au>Adelson, Martin E</au><au>Frese, Steven</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Species-Specific Analysis of Bacterial Vaginosis-Associated Bacteria</atitle><jtitle>Microbiology spectrum</jtitle><addtitle>Microbiol Spectr</addtitle><date>2023-08-17</date><risdate>2023</risdate><volume>11</volume><issue>4</issue><spage>e0467622</spage><epage>e0467622</epage><pages>e0467622-e0467622</pages><issn>2165-0497</issn><eissn>2165-0497</eissn><abstract>Vaginal dysbiosis in women reduces the abundance of
species and increases that of anaerobic fastidious bacteria. This dysbiotic condition in the vagina, called bacterial vaginosis (BV), can be symptomatic with odorous vaginal discharges or asymptomatic and affects a third of women of reproductive age. Three unclassified bacterial species designated BV-associated bacteria 1, 2, and 3 (BVAB-1, -2, and -3) in 2005 were found to be highly preponderant in the vagina of females with BV. Here, we used sequence homology and phylogenetics analyses to identify the actual species of BVAB-1, -2, and -3 and found BVAB-1 to be
genomosp. BVAB-1, BVAB-2 to be
bacterium strain CHIC02, and BVAB-3 to be Mageeibacillus indolicus, respectively. These are anaerobic and uncultured species that can be identified only through metagenomics. Long-read sequencing of BV specimens can also enable a genomic reassembly of these species' genomes from metagenomes. Species-specific identification of these pathogens and the availability of their genomes from assembled metagenomes will advance our understanding of their biology, facilitate the design of sensitive diagnostics and drugs, and enhance the treatment of BV.
For many years since 2005, BVAB, an important pathogen of the female vaginal tract that is associated with BV, has been identified using PCR without knowing its actual species. Without a full genome of these pathogens, a better understanding of their pathogenicity, treatment, resistance, and diagnostics cannot be reached. In this analysis, we use the DNA of BVAB-1, -2, and -3 to determine their actual species to enhance further research into their pathogenicity, resistance, diagnosis, and treatment.</abstract><cop>United States</cop><pub>American Society for Microbiology</pub><pmid>37347202</pmid><doi>10.1128/spectrum.04676-22</doi><orcidid>https://orcid.org/0000-0002-9508-984X</orcidid><orcidid>https://orcid.org/0000-0001-9912-1539</orcidid><orcidid>https://orcid.org/0000-0002-1147-6325</orcidid><oa>free_for_read</oa></addata></record> |
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title | Species-Specific Analysis of Bacterial Vaginosis-Associated Bacteria |
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