Identification of Clinical Immunological Determinants in Asymptomatic VL and Post Kala-azar Dermal Leishmaniasis Patients

• Published in: Iranian journal of parasitology 2018-10, Vol.13 (4), p.541-548
• Main Authors: Singh, Ashish Kumar, DAS, Vidya Nand Rabi, Amit, Ajay, Dikhit, Manas R, Mahantesh, Vijaya, Kumar, Akhilesh, Pandey, Raj Kishore, Naryan, Shyam, Singh, Bipin K, Pandey, Krishna, Verma, Neena, DAS, Pradeep, Bimal, Sanjiva
• Format: Article
• Language: English
• Subjects: Asymptomatic infection; Cytokine; Cytokines; Direct agglutination test; Health care; IFN-gamma; Immunology; Interleukin-10; Original; Parasitic diseases; Visceral leishmaniasis
• ISSN: 1735-7020; 2008-238X

Visceral Leishmaniasis (VL) caused by protozoa belonging to the genus usually have anthroponotic mode of transmission and is issue of great public health importance in Indian subcontinent. Asymptomatic cases of VL and PKDL are subject of keen interest to find their role in the transmission of VL in epidemic areas. We evaluated the immunological cytokine determinants expressed in most clinical suspects of asymptomatic VL and PKDL (IL-10, IFN-γ, and TNF-α). Eighty-four participants were included at RMRIMS, Patna, India in 2016-17 out of which 64 asymptomatic individual positive for rK-39, without sign and symptoms of VL; 15 PKDL patient's with past history of VL and 5 endemic healthy subjects were recruited from VL endemic areas. DAT and quantitative assessment of plasma cytokines was determined from the blood samples collected in a plain and sodium-EDTA vacutainer respectively from the subjects. The mean level of IL-10 in DAT of asymptomatic VL and PKDL was significantly higher than endemic healthy (