Cryptosporidium and Giardia in Biogas Wastewater: Management of Manure Livestock and Hygiene Aspects Using Influent, Effluent, Sewage Canal Samples, Vegetable, and Soil Samples

• Published in: Pathogens (Basel) 2022-01, Vol.11 (2), p.174
• Main Authors: Tram, Nguyen Thuy, Phuc, Pham Duc, Phi, Nguyen Hong, Trang, Le Thi, Nga, Tang Thi, Ha, Hoang Thi Thu, Cam, Phung Dac, Canh, Tran Quang, Karanis, Panagiotis
• Format: Article
• Language: English
• Subjects: Agricultural production; Agriculture; Alternative energy sources; Biogas; biogas wastewater; Canals; Clean technology; Cryptosporidium; Cysts; detection; Diarrhea; E coli; Effluents; Farmers; Farms; Gardens & gardening; Giardia; Health risk assessment; Health risks; Households; Hygiene; Livestock; Manures; Microorganisms; Oocysts; Parasites; Pollution; Questionnaires; Receiving waters; Risk analysis; Risk factors; Sanitation; Sewage; Sewage effluents; Soil contamination; Soil investigations; Statistical methods; Vegetables; Vietnam; Waste recycling; Wastewater; Watercourses
• ISSN: 2076-0817; 2076-0817
• DOI: 10.3390/pathogens11020174

and are two water- and foodborne protozoan parasites that can cause diarrheal diseases. Poor microbial quality, sanitation conditions, and hygiene practices at exposure to biogas wastewater are important risk factors for human and animal infection. This study highlights the presence and level of both parasites in the environment in relation to biogas waste reuse in Vietnam. A total of 239 samples were collected from different types of samples in the studied districts in Bac Giang province in 2020 via direct immunofluorescent detection to study the occurrence of spp. and spp. (oo)cysts. Among the samples, was found in 19 (7.9%) with concentration from 1.10 to 3.10 oocysts/100 mL, while in 40 (16.7%) with concentration from 1.10 to 2.10 cysts/100 mL, respectively. In detail, the results show that the percentages of positive detection of spp. and spp. in influent, effluent, sewage canal, and vegetables were 13.1% (11/84), 6.0% (5/83), 15.4% (2/13) and 5.9% (1/17) and 26.2% (22/84), 7.2% (6/83), 7.7% (1/13) and 5.9% (1/17), respectively. The results show a trend of decreasing and densities, without statistical significance. Although these parasites decreased after biogas treatment, the remaining loads observed in biogas effluent can reach the watercourses and soil receiving it. Further investigations are needed to contribute to a general understanding of the risk of protozoan parasites, as well as strategies to control and reduce the contamination of environmental water sources and plants and reduce the burden of the pathogens in biogas wastewater in Vietnam.