Quantification of cell cycle re-entry during dedifferentiation of primary adipocytes in vitro

Dedifferentiated adipose tissue (DFAT) has been proposed as a promising source of patient-specific multipotent progenitor cells (MPPs). During induced dedifferentiation, adipocytes exhibit profound gene expression and cell morphology changes. However, dedifferentiation of post-mitotic cells is expec...

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Bibliographic Details
Published in:Adipocyte 2024-12, Vol.13 (1), p.2376571
Main Author: Bielczyk-Maczynska, Ewa
Format: Article
Language:English
Subjects:
Adipocyte
Adipocytes - cytology
Adipocytes - metabolism
Adipose Tissue - cytology
Adipose Tissue - metabolism
Animals
Cell Cycle
Cell Dedifferentiation
Cell Differentiation
Cell Proliferation
Cells, Cultured
dedifferentiation
DFAT cells
lineage tracing
Mice
Multipotent Stem Cells - cytology
Multipotent Stem Cells - metabolism
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Summary:Dedifferentiated adipose tissue (DFAT) has been proposed as a promising source of patient-specific multipotent progenitor cells (MPPs). During induced dedifferentiation, adipocytes exhibit profound gene expression and cell morphology changes. However, dedifferentiation of post-mitotic cells is expected to enable proliferation, which is critical if enough MPPs are to be obtained. Here, lineage tracing was employed to quantify cell proliferation in mouse adipocytes subjected to a dedifferentiation-inducing protocol commonly used to obtain DFAT cells. No evidence of cell proliferation in adipocyte-derived cells was observed, in contrast to the robust proliferation of non-adipocyte cells present in adipose tissue. We conclude that proliferative MPPs derived using the ceiling culture method most likely arise from non-adipocyte cells in adipose tissue.
ISSN:2162-3945
2162-397X
2162-397X
DOI:10.1080/21623945.2024.2376571