SWATH-MS insights on sodium butyrate effect on mAbs production and redox homeostasis in CHO cells

Sodium butyrate (NaBu), well-known as a histone deacetylase inhibitor and for its capacity to impede cell growth, can enhance the production of a specific protein, such as an antibody, in recombinant Chinese hamster ovary (CHO) cell cultures. In this study, two CHO cell lines, namely K1 and DG44, al...

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Published in:AMB Express 2024-12, Vol.14 (1), p.140-18, Article 140
Main Authors: Galli, Mauro, Liu, Lillian Chia-Yi, Sim, Kae Hwan, Kok, Yee Jiun, Wongtrakul-Kish, Katherine, Nguyen-Khuong, Terry, Tate, Stephen, Bi, Xuezhi
Format: Article
Language:English
Subjects:
Apoptosis
Biomedical and Life Sciences
Biosynthesis
Biotechnology
Butyric acid
Cell culture
Cell cycle
Cell growth
Cell lines
CHO cells
Gene expression
Glycosylation
Histone deacetylase
Homeostasis
Life Sciences
Lipid metabolism
Lipids
mAbs
Manufacturers
Microbial Genetics and Genomics
Microbiology
Mitosis
Monoclonal antibodies
Original
Original Article
Oxidation
Peptides
Productivity
Proteins
Proteomes
R&D
Redox
Research & development
Sodium
Sodium butyrate
SWATH-MS
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Summary:Sodium butyrate (NaBu), well-known as a histone deacetylase inhibitor and for its capacity to impede cell growth, can enhance the production of a specific protein, such as an antibody, in recombinant Chinese hamster ovary (CHO) cell cultures. In this study, two CHO cell lines, namely K1 and DG44, along with their corresponding mAb-producing lines, K1-Pr and DG44-Pr, were cultivated with or without NaBu. A SWATH-based profiling method was employed to analyze the proteome. Cells cultured in the presence of NaBu exhibited a reduction in mitosis and gene expression, supported by their culture data demonstrating growth inhibition. The presence of NaBu corresponded to upregulation of intracellular trafficking and secretion pathways, aligned with an observed increase in mAb production, and was associated with an elevated glycosylation pathway and a slight alteration in the glycosylation profile of the mAbs. Increased fatty acid oxidation, redox interactions, and lipid biosynthesis were also observed and are likely attributable to the metabolism of NaBu. A comprehensive understanding of the systemic effects of NaBu will facilitate the discovery of strategies to enhance or prolong the productivity of CHO cells. Graphical Abstract
ISSN:2191-0855
2191-0855
DOI:10.1186/s13568-024-01807-z