Phages from Genus Bruynoghevirus and Phage Therapy: Pseudomonas Phage Delta Case

The applicability and safety of bacteriophage Delta as a potential anti- agent belonging to genus (family ) was characterised. Phage Delta belongs to the species , which has been described as a temperate, with sites at the end of the genome. The phage Delta possesses a genome of 45,970 bp that encod...

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Bibliographic Details
Published in:Viruses 2021-09, Vol.13 (10), p.1965
Main Authors: Knezevic, Petar, Petrovic Fabijan, Aleksandra, Gavric, Damir, Pejic, Jovana, Doffkay, Zsolt, Rakhely, Gábor
Format: Article
Language:English
Subjects:
Algorithms
Allergens
Antibiotic resistance
antibiotic resistance genes
Antibiotics
Asparagine
Aspartic acid
Bacteria
bacterial virulence factors
bacteriophage insensitive mutants (BIM)
Bacteriophages - genetics
Bacteriophages - isolation & purification
Biofilms
Caudovirales - genetics
DNA, Viral - genetics
Drug resistance
Enzymes
genome ends
Genome, Viral - genetics
Genomes
Genomics
Host range
Host Specificity - genetics
Lysogeny
Maximum likelihood method
Multiplicity of infection
Phages
Phylogenetics
Plaques
Podoviridae - genetics
Podoviridae - metabolism
Proline
Proteins
Pseudomonas
Pseudomonas aeruginosa - virology
Pseudomonas phage Delta
Pseudomonas Phages - genetics
Pseudomonas virus PaP3
Toxins
tRNA Asp
tRNA Pro
Virulence
Virulence factors
Viruses
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Description
Summary:The applicability and safety of bacteriophage Delta as a potential anti- agent belonging to genus (family ) was characterised. Phage Delta belongs to the species , which has been described as a temperate, with sites at the end of the genome. The phage Delta possesses a genome of 45,970 bp that encodes tRNA for proline (Pro), aspartic acid (Asp) and asparagine (Asn) and does not encode any known protein involved in lysogeny formation or persistence. Analysis showed that phage Delta has 182 bp direct terminal repeats at the end of genome and lysogeny was confirmed, neither upon infection at low nor at high multiplicity of infection (MOI). The turbid plaques that appear on certain host lawns can result from bacteriophage insensitive mutants that occur with higher frequency (10 ). In silico analysis showed that the genome of Delta phage does not encode any known bacterial toxin or virulence factor, determinants of antibiotic resistance and known human allergens. Based on the broad host range and high lytic activity against planktonic and biofilm cells, phage Delta represents a promising candidate for phage therapy.
ISSN:1999-4915
1999-4915
DOI:10.3390/v13101965