Common polymorphisms in GSTM1, GSTT1, GSTP1, GSTA1 and susceptibility to colorectal cancer in the Central European population

• Published in: European journal of medical research 2012-06, Vol.17 (1), p.17-17, Article 17
• Main Authors: Hezova, Renata, Bienertova-Vasku, Julie, Sachlova, Milana, Brezkova, Veronika, Vasku, Anna, Svoboda, Marek, Radová, Lenka, Kiss, Igor, Vyzula, Rostislav, Slaby, Ondrej
• Format: Article
• Language: English
• Subjects: Adult; Aged; Alleles; Case-Control Studies; Colorectal cancer; Colorectal Neoplasms - enzymology; Colorectal Neoplasms - epidemiology; Colorectal Neoplasms - genetics; Czech Republic - epidemiology; Disease susceptibility; Disease Susceptibility - enzymology; European Continental Ancestry Group - genetics; Female; Gene Frequency; Gene-Environment Interaction; Genes; Genetic aspects; Genetic Association Studies; Genetic polymorphisms; Genetic Predisposition to Disease; Genetic Testing - methods; Glutathione S-Transferase pi - genetics; Glutathione transferase; Glutathione Transferase - genetics; Goods and services tax; GSTA1; GSTM1; GSTP1; GSTT1; Humans; Incidence; Male; Middle Aged; Polymorphism; Polymorphism, Genetic; Polymorphism, Restriction Fragment Length; Real-Time Polymerase Chain Reaction; Risk Factors; Thiols; Xenobiotics
• ISSN: 2047-783X; 0949-2321; 2047-783X
• DOI: 10.1186/2047-783X-17-17

Central Europe presents with the highest incidence of sporadic colorectal cancer (CRC) worldwide. As sporadic CRC represents a typical multifactorial disease, it is characterized by intense interaction of the genetic background with the environment. Glutathione S-transferases could act as attractive susceptibility genes for CRC, as they are directly involved in conjugation between glutathione and chemotherapeutics, environmental pollutants and a wide spectrum of xenobiotics. In this study, we investigated associations of polymorphisms in glutathione S-transferases (GSTs) genes, that is GSTA1, GSTT1, GSTM1 and GSTP1, with CRC in a total of 197 cases and 218 controls originating from the Czech Central European population. Polymorphisms were assessed by polymerase chain reaction/restriction fragment length polymorphism-based methods, allele-specific multiplex and allelic discrimination by real-time polymerase chain reaction. None of investigated polymorphisms showed any associations with CRC, with the exception of GSTP1; where the heterozygote genotype Ile105Val was associated with decreased risk of CRC (P = 0.043). The frequencies observed in our study are in accordance with those from other European Caucasian populations. Based on our studies, examined variability in GST genes is not a major determinant of CRC susceptibility in the Central European population.