Identification of Blood Circular RNAs as Potential Biomarkers for Acute Ischemic Stroke

Many hospitals lack facilities for accurate diagnosis of acute ischemic stroke (AIS). Circular RNA (circRNA) is highly expressed in the brain and is closely associated with stroke. In this study, we examined whether the blood-borne circRNAs could be promising candidates as adjunctive diagnostic biom...

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Bibliographic Details
Published in:Frontiers in neuroscience 2020-02, Vol.14, p.81-81
Main Authors: Lu, Dan, Ho, Eric S, Mai, Hongcheng, Zang, Jiankun, Liu, Yanfang, Li, Yufeng, Yang, Bing, Ding, Yan, Tsang, Chi Kwan, Xu, Anding
Format: Article
Language:English
Subjects:
acute ischemic stroke
Bioinformatics
bioinformatics analysis
Biomarkers
Blood
cerebral ischemia
Circular RNA
Diagnosis
Experiments
Extracellular matrix
Gene expression
Hybridization
Ischemia
Laboratory animals
Neuroscience
Physiology
Ribonucleic acid
RNA
Signal transduction
Stroke
Veins & arteries
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Summary:Many hospitals lack facilities for accurate diagnosis of acute ischemic stroke (AIS). Circular RNA (circRNA) is highly expressed in the brain and is closely associated with stroke. In this study, we examined whether the blood-borne circRNAs could be promising candidates as adjunctive diagnostic biomarkers and their pathophysiological roles after stroke. We profiled the blood circRNA expression in mice subjected to experimental focal cerebral ischemia and validated the selected circRNAs in AIS patients. We demonstrated that 128, 198, and 789 circRNAs were significantly altered at 5 min, 3 h, and 24 h after ischemic stroke, respectively. Our bioinformatics analysis revealed that the circRNA-targeted genes were associated with the Hippo signaling pathway, extracellular matrix-receptor interaction, and fatty acid metabolism at 5 min, 3 h and 24 h after ischemic stroke, respectively. We verified that many of these circRNAs existed in the mouse brain. Furthermore, we found that most of the predicted circRNA-miRNA interactions apparently exhibited functional roles in terms of regulation of their target gene expression in the brain. We also verified that many of these mouse circRNAs were conserved in human. Finally, we found that circBBS2 and circPHKA2 were differentially expressed in the blood of AIS patients. These results demonstrate that blood circRNAs may serve as potential biomarkers for AIS diagnosis and reveal the pathophysiological responses in the brain after ischemic stroke.
ISSN:1662-4548
1662-453X
1662-453X
DOI:10.3389/fnins.2020.00081