Gold Nanocluster-Based Fluorometric Banoxantrone Assay Enabled by Photoinduced Electron Transfer

Monitoring the blood concentration of banoxantrone (AQ4N) is important to evaluate the therapeutic efficacy and side effects of this new anticancer prodrug during its clinical applications. Herein, we report a fluorescence method for AQ4N detection through the modulation of the molecule-like photoin...

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Bibliographic Details
Published in:Nanomaterials (Basel, Switzerland) Switzerland), 2022-05, Vol.12 (11), p.1861
Main Authors: Huang, Kai-Yuan, Weng, Wen-Hui, Huang, Xin, Huang, Hong-Xiang, Noreldeen, Hamada A A, Deng, Hao-Hua, Chen, Wei
Format: Article
Language:English
Subjects:
Anticancer properties
banoxantrone
Blood levels
Chitosan
Coulomb interaction
Dithiothreitol
Electrodes
Electron transfer
Experiments
Fluorescence
Gold
gold nanocluster
Luminescence quenching
Nanoclusters
Nanoparticles
Oxidation
photoinduced electron transfer
photoluminescence quenching
Sensors
Side effects
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Summary:Monitoring the blood concentration of banoxantrone (AQ4N) is important to evaluate the therapeutic efficacy and side effects of this new anticancer prodrug during its clinical applications. Herein, we report a fluorescence method for AQ4N detection through the modulation of the molecule-like photoinduced electron transfer (PET) behavior of gold nanoclusters (AuNCs). AQ4N can electrostatically bind to the surface of carboxylated chitosan (CC) and dithiothreitol (DTT) co-stabilized AuNCs and quench their fluorescence via a Coulomb interaction-accelerated PET process. Under optimized experimental conditions, the linear range of AQ4N is from 25 to 200 nM and the limit of detection is as low as 5 nM. In addition, this assay is confirmed to be reliable based on its successful use in AQ4N determination in mouse plasma samples. This work offers an effective strategy for AQ4N sensing based on fluorescent AuNCs and widens the application of AuNCs in clinical diagnosis and pharmaceutical analysis.
ISSN:2079-4991
2079-4991
DOI:10.3390/nano12111861