Acute phase reaction to lipopolysaccharide-induced mastitis in early lactation dairy cows fed nitrogenic, glucogenic, or lipogenic diets

• Published in: Journal of dairy science 2023-12, Vol.106 (12), p.9879-9891
• Main Authors: Jermann, P M, Wagner, L A, Fritsche, D, Gross, J J, Wellnitz, O, Bruckmaier, R M
• Format: Article
• Language: English
• Subjects: acute phase proteins; Acute-Phase Reaction - metabolism; Acute-Phase Reaction - veterinary; amyloid; Animals; blood serum; Cattle; Cattle Diseases - metabolism; crude protein; dairy science; diet; Diet - veterinary; early lactation; family; Female; gene expression; Glucose - metabolism; glucose transporters; haptoglobins; heat shock proteins; immune system; integrins; interleukin-8; Interleukin-8 - metabolism; Lactation - physiology; leukocytes; lipopolysaccharides; Lipopolysaccharides - pharmacology; mastitis; Mastitis - metabolism; Mastitis - veterinary; metabolism; milk; Milk - metabolism; reverse transcriptase polymerase chain reaction; RNA, Messenger - metabolism; Serum Amyloid A Protein - metabolism; standard deviation; tumor necrosis factors
• ISSN: 0022-0302; 1525-3198
• DOI: 10.3168/jds.2023-23582

The availability of certain macronutrients is likely to influence the capacity of the immune system. Therefore, we investigated the acute phase response to intramammary (i.mam.) lipopolysaccharide (LPS) in dairy cows fed a nitrogenic diet (n = 10) high in crude protein, a glucogenic diet (n = 11) high in carbohydrates and glucogenic precursors, or a lipogenic diet (n = 11) high in lipids. Thirty-two dairy cows were fed one of the dietary concentrates directly after calving until the end of trial at 27 ± 3 days in milk (mean ± standard deviation). In wk 3 of lactation, 20 µg of LPS was i.mam. injected in one quarter, and sterile NaCl (0.9%) in the contralateral quarter. Milk samples of the LPS-challenged and control quarter were taken hourly from before (0 h) until 9 h after LPS challenge and analyzed for milk amyloid A (MAA), haptoglobin (HP), and IL-8. In addition, blood samples were taken in the morning, and composite milk samples at morning and evening milkings, from 1 d before until 3 d after LPS challenge, and again on d 9, to determine serum amyloid A (SAA) and HP in blood, and MAA and HP in milk. The mRNA abundance of various immunological and metabolic factors in blood leukocytes was quantified by quantitative reverse-transcription PCR from samples taken at -18, -1, 6, 9, and 23 h relative to LPS application. The dietary concentrates did not affect any of the parameters in blood, milk, and leukocytes. The IL-8 was increased from 2 h, HP from 2 to 3 h, and MAA from 6 h relative to the LPS administration in the milk of the challenged quarter and remained elevated until 9 h. The MAA and HP were also increased at 9 h after LPS challenge in whole-udder composite milk, whereas HP and SAA in blood were increased only after 23 h. All 4 parameters were decreased again on d 9. Similar for all groups, the mRNA abundance of HP and the heat shock protein family A increased after the LPS challenge, whereas the mRNA expression of the tumor necrosis factor α and the leukocyte integrin β 2 subunit (CD18) were decreased at 6 h after LPS challenge. The glucose transporter (GLUT)1 mRNA abundance decreased after LPS, whereas that of the GLUT3 increased, and that of the GLUT4 was not detectable. The mRNA abundance of GAPDH was increased at 9 h after LPS and remained elevated. The acute phase protein response was detected earlier in milk compared with blood indicating mammary production. However, immunological responses to LPS were not affected by the availability of spe