Mucosal Heterologous Prime/Boost Vaccination Induces Polyfunctional Systemic Immunity, Improving Protection Against Trypanosoma cruzi

There are several unmet needs in modern immunology. Among them, vaccines against parasitic diseases and chronic infections lead. , the causative agent of Chagas disease, is an excellent example of a silent parasitic invasion that affects millions of people worldwide due to its progression into the s...

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Bibliographic Details
Published in:Frontiers in immunology 2020-02, Vol.11, p.128-128
Main Authors: Sanchez Alberti, Andrés, Bivona, Augusto E, Matos, Marina N, Cerny, Natacha, Schulze, Kai, Weißmann, Sebastian, Ebensen, Thomas, González, Germán, Morales, Celina, Cardoso, Alejandro C, Cazorla, Silvia I, Guzmán, Carlos A, Malchiodi, Emilio L
Format: Article
Language:English
Subjects:
Animals
Anti-Trypanosoma cruzi vaccine
CD4-Positive T-Lymphocytes
CD8-Positive T-Lymphocytes - immunology
Chagas disease
cyclic-di-AMP
Cytokines - metabolism
Female
Immunity - immunology
Immunization, Secondary
Immunology
Male
Mice
Models, Animal
neglected tropical disease
prime-boost vaccine
Protozoan Vaccines - immunology
Traspain
Treatment Outcome
Trypanosoma cruzi - immunology
Vaccination - methods
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Summary:There are several unmet needs in modern immunology. Among them, vaccines against parasitic diseases and chronic infections lead. , the causative agent of Chagas disease, is an excellent example of a silent parasitic invasion that affects millions of people worldwide due to its progression into the symptomatic chronic phase of infection. In search for novel vaccine candidates, we have previously introduced Traspain, an engineered trivalent immunogen that was designed to address some of the known mechanisms of immune evasion. Here, we analyzed its performance in different DNA prime/protein boost protocols and characterized the systemic immune response associated with diverse levels of protection. Formulations that include a STING agonist, like c-di-AMP in the boost doses, were able to prime a Th1/Th17 immune response. Moreover, comparison between them showed that vaccines that were able to prime polyfunctional cell-mediated immunity at the CD4 and CD8 compartment enhanced protection levels in the murine model. These findings contribute to a better knowledge of the desired vaccine-elicited immunity against and promote the definition of a vaccine correlate of protection against the infection.
ISSN:1664-3224
1664-3224
DOI:10.3389/fimmu.2020.00128