Dynamics of maternal gene expression in Rhodnius prolixus

The study of developmental processes in Rhodnius prolixus has recently advanced with the sequencing of the genome. In this work, we analyze the maternal gene expression driving oogenesis and early embryogenesis in R. prolixus . We examined the transcriptional profile of mRNAs to establish the genes...

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Bibliographic Details
Published in:Scientific reports 2022-04, Vol.12 (1), p.6538-6538, Article 6538
Main Authors: Pascual, Agustina, Rivera-Pomar, Rolando
Format: Article
Language:English
Subjects:
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631/208
Animals
Blastoderm
Egg laying
Embryogenesis
Embryonic Development - genetics
Embryonic growth stage
Female
Gene Expression
Genomes
Humanities and Social Sciences
Hybridization
multidisciplinary
Oogenesis
Oogenesis - genetics
Ovaries
Rhodnius - genetics
Rhodnius - metabolism
Rhodnius prolixus
RNA Interference
RNA-mediated interference
Science
Science (multidisciplinary)
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Summary:The study of developmental processes in Rhodnius prolixus has recently advanced with the sequencing of the genome. In this work, we analyze the maternal gene expression driving oogenesis and early embryogenesis in R. prolixus . We examined the transcriptional profile of mRNAs to establish the genes expressed across the ovary, unfertilized eggs and different embryonic stages of R. prolixus until the formation of the germ band anlage (0, 12, 24, and 48 h post egg laying). We identified 81 putative maternal and ovary-related genes and validated their expression by qRT-PCR. We validate the function of the ortholog gene Bicaudal-D ( Rp-BicD ) by in situ hybridization and parental RNAi. Consistent with a role in oogenesis and early development of R. prolixus , we show that lack of Rp-BicD does not significantly affect oogenesis but impairs the formation of the blastoderm. Based on our findings, we propose three times of action for maternal genes during oogenesis and embryogenesis in R. prolixus .
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-022-09874-7