Detection of the Phenicol–Oxazolidinone Resistance Gene poxtA in Enterococcus faecium and Enterococcus faecalis from Food-Producing Animals during 2008–2018 in Korea

We aimed to investigate the presence of the phenicol–oxazolidinone resistance gene poxtA in linezolid-resistant enterococci from food-producing animals and analyze its molecular characteristics. We collected 3941 Enterococcus faecium and 5088 E. faecalis isolates from all provinces of South Korea fr...

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Bibliographic Details
Published in:Microorganisms (Basel) 2020-11, Vol.8 (11), p.1839
Main Authors: Moon, Dong-Chan, Kim, Mi-Hyun, Kang, Hee-Young, Kim, Su-Jeong, Choi, Ji-Hyun, Mechesso, Abraham-Fikru, Yoon, Soon-Seek, Lim, Suk-Kyung
Format: Article
Language:English
Subjects:
Animals
Antimicrobial agents
Antimicrobial resistance
Cattle
clonal complex 17
Conjugation
Drug resistance
Electrophoresis
Enterococcus
Enterococcus faecium
Feces
Food
Gel electrophoresis
Genes
Laboratories
Linezolid
linezolid resistance
Monitoring systems
Multilocus sequence typing
Mutation
poxtA
Proteins
Pulsed-field gel electrophoresis
Ribosomal protein L4
Risk reduction
Staphylococcus infections
Veterinary medicine
Zoonoses
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Summary:We aimed to investigate the presence of the phenicol–oxazolidinone resistance gene poxtA in linezolid-resistant enterococci from food-producing animals and analyze its molecular characteristics. We collected 3941 Enterococcus faecium and 5088 E. faecalis isolates from all provinces of South Korea from 2008 to 2018. We found linezolid resistance in 0.79% (94/3941) of E. faecium and 1.22% (62/5088) of E. faecalis isolates. Overall, 23.1% (36/156) of the linezolid-resistant isolates had the poxtA gene, including 31 E. faecium and five E. faecalis isolates. The poxtA-positive enterococci were mainly isolated from chicken (86.1%; 26/36). Fifteen poxtA-harboring isolates co-carried another linezolid-resistance gene, optrA. Eight E. faecium isolates had an N130K mutation in the ribosomal protein L4, while no mutations were observed in E. faecalis isolates. The poxtA gene was transferred into 10 enterococci by conjugation. Multi-locus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE) analysis indicated that poxtA-carrying isolates were heterogeneous. Three E. faecium isolates belonged to CC17 (ST32, ST121, and ST491). To our knowledge, this is the first report on the poxtA gene in Korea. Prudent use of antimicrobials and active surveillance on antimicrobial resistance are urgently needed to reduce the risk of dissemination of the linezolid-resistant isolates in humans and animals.
ISSN:2076-2607
2076-2607
DOI:10.3390/microorganisms8111839