Continuous Quadrupole Magnetic Separation of Islets during Digestion Improves Purified Porcine Islet Viability

Islet transplantation (ITx) is an emerging and promising therapy for patients with uncontrolled type 1 diabetes. The islet isolation and purification processes require exposure to extended cold ischemia, warm-enzymatic digestion, mechanical agitation, and use of damaging chemicals for density gradie...

Full description

Saved in:
Bibliographic Details
Published in:Journal of diabetes research 2016-01, Vol.2016 (2016), p.1-10
Main Authors: Todd, Paul W., Balamurugan, Appakalai N., Hering, Bernhard J., Papas, Klearchos K., Hanley, Thomas R., Mueller, Kate R., Kitzmann, Jennifer P., Scott III, William E., Rizzari, Michael D., Suszynski, Thomas M., Kumar Sajja, Venkata Sunil, Weegman, Bradley P., Kennedy, David J.
Format: Article
Language:English
Subjects:
Animals
Centrifugation, Density Gradient
Deoxyribonucleic acid
DNA
Enzymes
Hypoxia
Insulin
Ischemia
Islets of Langerhans
Islets of Langerhans Transplantation
Magnets
Methods
Morphology
Pancreas
Quality
Shear stress
Success
Swine
Transplants & implants
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Islet transplantation (ITx) is an emerging and promising therapy for patients with uncontrolled type 1 diabetes. The islet isolation and purification processes require exposure to extended cold ischemia, warm-enzymatic digestion, mechanical agitation, and use of damaging chemicals for density gradient separation (DG), all of which reduce viable islet yield. In this paper, we describe initial proof-of-concept studies exploring quadrupole magnetic separation (QMS) of islets as an alternative to DG to reduce exposure to these harsh conditions. Three porcine pancreata were split into two parts, the splenic lobe (SPL) and the combined connecting/duodenal lobes (CDL), for paired digestions and purifications. Islets in the SPL were preferentially labeled using magnetic microparticles (MMPs) that lodge within the islet microvasculature when infused into the pancreas and were continuously separated from the exocrine tissue by QMS during the collection phase of the digestion process. Unlabeled islets from the CDL were purified by conventional DG. Islets purified by QMS exhibited significantly improved viability (measured by oxygen consumption rate per DNA, p
ISSN:2314-6745
2314-6753
DOI:10.1155/2016/6162970