Features of polymorphism of calpastatin and somatotropin genes in young sheep, obtained from crossing ewes of Kalmyk fat-rumped sheeps and dorper rams

• Published in: E3S Web of Conferences 2020-01, Vol.175, p.3020
• Main Authors: Pogodaev, Vladimir, Aduchiev, Bator, Kononova, Lydia, Aslanukova, Maya, Kardanova, Irina
• Format: Article
• Language: English
• Subjects: Alleles; Calpastatin; Gene frequency; Gene polymorphism; Genes; Genotype & phenotype; Genotypes; Growth hormones; Polymorphism; Sheep
• ISSN: 2267-1242; 2555-0403; 2267-1242
• DOI: 10.1051/e3sconf/202017503020

The article presents the study of polymorphism of the CAST and GH genes, which determine the features of the manifestation of the productive and biological characteristics of sheep with ½ Kalmyk + ½ Dorper blood system. Calpastatin gene polymorphism represented by alleles M and N, whose frequency was 0,65 and 0,35; genotypes MM, MN – 30 and 70% accordingly. The desired NN genotype has not been identified. A relatively uniform incidence of allele N (0,35) CAST gene and B (0,40) GH was established, what contributed to an almost equal distribution of allele frequencies М (0,65) and А (0,60) genes of calpastatin and somatotropin accordingly. The frequency of heterozygous genotypes occurrence by CAST gene composed 0,7. By GH gene the following distribution of genotype frequencies is observed. Frequency of occurrence homozygous АА and heterozygous AB genotypes was equal to and is 0,4, wherein the frequency of occurrence advised homozygous ВВ genotype was 0,2. Among the animals studied, sheep with a complex genotype are most common CAST MN GHAB (40 %). The amount of percent CAST MM GHAA and CAST MN GHAA is in 22,2%. 10% is for genotypes CAST MM GHBB and CAST MN GHBB.