Real-time reverse transcription-polymerase chain reaction assay for SARS-associated coronavirus

A real-time reverse transcription-polymerase chain reaction (RT-PCR) assay was developed to rapidly detect the severe acute respiratory syndrome-associated coronavirus (SARS-CoV). The assay, based on multiple primer and probe sets located in different regions of the SARS-CoV genome, could discrimina...

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Bibliographic Details
Published in:Emerging infectious diseases 2004-02, Vol.10 (2), p.311-316
Main Authors: Emery, Shannon L, Erdman, Dean D, Bowen, Michael D, Newton, Bruce R, Winchell, Jonas M, Meyer, Richard F, Tong, Suxiang, Cook, Byron T, Holloway, Brian P, McCaustland, Karen A, Rota, Paul A, Bankamp, Bettina, Lowe, Luis E, Ksiazek, Tom G, Bellini, William J, Anderson, Larry J
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Canada
Chlorocebus aethiops
Coronavirus
DNA Primers - genetics
DNA, Viral - genetics
Humans
Real-Time PCR
Reproducibility of Results
Reverse Transcriptase Polymerase Chain Reaction - statistics & numerical data
RNA, Viral - genetics
RNA, Viral - isolation & purification
RT-PCR
SARS
SARS coronavirus
SARS Virus - genetics
SARS Virus - isolation & purification
Sensitivity and Specificity
Severe Acute Respiratory Syndrome - diagnosis
Severe Acute Respiratory Syndrome - epidemiology
Severe Acute Respiratory Syndrome - virology
Thailand
Vero Cells
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Description
Summary:A real-time reverse transcription-polymerase chain reaction (RT-PCR) assay was developed to rapidly detect the severe acute respiratory syndrome-associated coronavirus (SARS-CoV). The assay, based on multiple primer and probe sets located in different regions of the SARS-CoV genome, could discriminate SARS-CoV from other human and animal coronaviruses with a potential detection limit of
ISSN:1080-6040
1080-6059
DOI:10.3201/eid1002.030759