FXYD8, a Novel Regulator of Renal Na + /K + -ATPase in the Euryhaline Teleost, Tetraodon nigroviridis

FXYD proteins are important regulators of Na /K -ATPase (NKA) activity in mammals. As an inhabitant of estuaries, the pufferfish ( ) responds to ambient salinity changes with efficient osmoregulation, including alterations in branchial, and renal NKA activities. Previous studies on teleostean FXYDs...

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Bibliographic Details
Published in:Frontiers in physiology 2017-08, Vol.8, p.576-576
Main Authors: Wang, Pei-Jen, Yang, Wen-Kai, Lin, Chia-Hao, Hwang, Hau-Hsuan, Lee, Tsung-Han
Format: Article
Language:English
Subjects:
FXYD8
kidney
Na+/K+-ATPase
Physiology
pufferfish
salinity
Tetraodon nigroviridis
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Summary:FXYD proteins are important regulators of Na /K -ATPase (NKA) activity in mammals. As an inhabitant of estuaries, the pufferfish ( ) responds to ambient salinity changes with efficient osmoregulation, including alterations in branchial, and renal NKA activities. Previous studies on teleostean FXYDs have mainly focused on the expression and potential functions of FXYD proteins in gills. The goal of the present study was to elucidate the potential role of FXYD8, a member of the fish FXYD protein family, in the modulation of NKA activity in the kidneys of this euryhaline pufferfish by using molecular, biochemical, and physiological approaches. The results demonstrate that FXYD8 (TnFXYD8) interacts with NKA in renal tubules. Meanwhile, the protein expression of renal TnFXYD8 was found to be significantly upregulated in hyperosmotic seawater-acclimated pufferfish. Moreover, overexpression of TnFXYD8 in oocytes decreased NKA activity. Our results suggest the FXYD8 is able to modulate NKA activity through inhibitory effects upon salinity challenge. The present study further extends our understanding of the functions of FXYD proteins, the regulators of NKA, in vertebrates.
ISSN:1664-042X
1664-042X
DOI:10.3389/fphys.2017.00576