Recent Advances in the Heterologous Biosynthesis of Natural Products from Streptomyces

Streptomyces is a significant source of natural products that are used as therapeutic antibiotics, anticancer and antitumor agents, pesticides, and dyes. Recently, with the advances in metabolite analysis, many new secondary metabolites have been characterized. Moreover, genome mining approaches dem...

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Bibliographic Details
Published in:Applied sciences 2021-02, Vol.11 (4), p.1851
Main Authors: Pham, Van Thuy Thi, Nguyen, Chung Thanh, Dhakal, Dipesh, Nguyen, Hue Thi, Kim, Tae-Su, Sohng, Jae Kyung
Format: Article
Language:English
Subjects:
Antibiotics
Bioinformatics
Biosynthesis
Cloning
Deoxyribonucleic acid
DNA
Drugs
Gene clusters
Gene deletion
Genetic control
Genetic engineering
Genomes
Gram-positive bacteria
heterologous expression
Host selection
Mass spectrometry
Metabolism
Metabolites
Natural products
Peptides
Pesticides
Rodeos
Scientific imaging
secondary metabolite
Secondary metabolites
Streptomyces
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Summary:Streptomyces is a significant source of natural products that are used as therapeutic antibiotics, anticancer and antitumor agents, pesticides, and dyes. Recently, with the advances in metabolite analysis, many new secondary metabolites have been characterized. Moreover, genome mining approaches demonstrate that many silent and cryptic biosynthetic gene clusters (BGCs) and many secondary metabolites are produced in very low amounts under laboratory conditions. One strain many compounds (OSMAC), overexpression/deletion of regulatory genes, ribosome engineering, and promoter replacement have been utilized to activate or enhance the production titer of target compounds. Hence, the heterologous expression of BGCs by transferring to a suitable production platform has been successfully employed for the detection, characterization, and yield quantity production of many secondary metabolites. In this review, we introduce the systematic approach for the heterologous production of secondary metabolites from Streptomyces in Streptomyces and other hosts, the genome analysis tools, the host selection, and the development of genetic control elements for heterologous expression and the production of secondary metabolites.
ISSN:2076-3417
2076-3417
DOI:10.3390/app11041851