SlSERK3B Promotes Tomato Seedling Growth and Development by Regulating Photosynthetic Capacity

• Published in: International journal of molecular sciences 2024-01, Vol.25 (2), p.1336
• Main Authors: Ding, Zhiqi, Yao, Yandong, Yao, Kangding, Hou, Xuemei, Zhang, Zhuohui, Huang, Yi, Wang, Chunlei, Liao, Weibiao
• Format: Article
• Language: English
• Subjects: brassinosteroids; Brassinosteroids - metabolism; Brassinosteroids - pharmacology; Carotenoids; Chlorophyll; Chlorophyll - metabolism; Enzymes; Genes; Genetic engineering; Genomes; Growth and Development; Kinases; Leaves; Photosynthesis; photosynthetic capacity; pigment content; Plant growth; Seedlings; SlSERK3B; Solanum lycopersicum - genetics; Tomatoes; virus-induced gene silencing
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms25021336

Brassinosteroids (BRs) are a group of polyhydroxylated steroids for plant growth and development, regulating numerous physiological and biochemical processes and participating in multi-pathway signaling in plants. 24-Epibrassinolide (EBR) is the most commonly used BR for the investigation of the effects of exogenous steroidal phytohormones on plant physiology. Although is considered a gene involved in the brassinosteroid (BR) signaling pathway, its specific role in plant growth and development has not been reported in detail. In this study, tomato ( L.) seedlings treated with 0.05 μmol L EBR showed a significant increase in plant height, stem diameter, and fresh weight, demonstrating that BR promotes the growth of tomato seedlings. EBR treatment increased the expression of the BR receptor gene , the co-receptor gene and its homologs , and . The gene was silenced by TRV-mediated virus-induced gene silencing (VIGS) technology. The results showed that both brassinolide (BL) content and BR synthesis genes were significantly up-regulated in TRV-SlSERK3B-infected seedlings compared to the control seedlings. In contrast, plant height, stem diameter, fresh weight, leaf area and total root length were significantly reduced in silenced plants. These results suggest that silencing may affect BR synthesis and signaling, thereby affecting the growth of tomato seedlings. Furthermore, the photosynthetic capacity of TRV- -infected tomato seedlings was reduced, accompanied by decreased photosynthetic pigment content chlorophyll fluorescence, and photosynthesis parameters. The expression levels of chlorophyll-degrading genes were significantly up-regulated, and carotenoid-synthesising genes were significantly down-regulated in TRV- -infected seedlings. In conclusion, silencing of inhibited BR signaling and reduced photosynthesis in tomato seedlings, and this correlation suggests that may be related to BR signaling and photosynthesis enhancement.