In Vitro Nonalcoholic Fatty Liver Disease Model Elucidating the Effect of Immune Environment on Disease Progression and Alleviation

Nonalcoholic fatty liver disease (NAFLD), which is a major cause of chronic liver disease, is characterized by fat accumulation in the liver. Existing models struggle to assess medication effects on liver function in the context of NAFLD’s unique inflammatory environment. We address this by developi...

Full description

Saved in:
Bibliographic Details
Published in:ACS omega 2024-06, Vol.9 (23), p.25094-25105
Main Authors: Kim, Inhye, Kyun, Mi-lang, Jung, Hyewon, Kwon, Ji-In, Kim, Jeongha, Kim, Ju-Kang, Lee, Yu Bin, Kwon, Young-In, Moon, Kyoung-Sik
Format: Article
Language:English
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites cdi_FETCH-LOGICAL-a383t-4b0e9f5f0622e0e7eeecedeab8a7abcba2b2fc3da772a9e836a3c64f077935253
container_end_page 25105
container_issue 23
container_start_page 25094
container_title ACS omega
container_volume 9
creator Kim, Inhye
Kyun, Mi-lang
Jung, Hyewon
Kwon, Ji-In
Kim, Jeongha
Kim, Ju-Kang
Lee, Yu Bin
Kwon, Young-In
Moon, Kyoung-Sik
description Nonalcoholic fatty liver disease (NAFLD), which is a major cause of chronic liver disease, is characterized by fat accumulation in the liver. Existing models struggle to assess medication effects on liver function in the context of NAFLD’s unique inflammatory environment. We address this by developing a 3D in vitro NAFLD model using HepG2 and THP-1 cells (mimicking liver and Kupffer cells) cocultured using transwell and hydrogel system. This mimics liver architecture and allows for manipulation of the immune environment. We demonstrate that the model recapitulates key NAFLD features: steatosis (induced by fatty acids), oxidative stress, inflammation, and impaired liver function embodying the interrelationship between NAFLD and the surrounding immune environment. This versatile model offers a valuable tool for preclinical NAFLD research by incorporating a disease-relevant immune environment.
doi_str_mv 10.1021/acsomega.4c02433
format article
fullrecord <record><control><sourceid>proquest_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_ab4cbfcf01564468b9352a3f56e67168</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_ab4cbfcf01564468b9352a3f56e67168</doaj_id><sourcerecordid>3069174500</sourcerecordid><originalsourceid>FETCH-LOGICAL-a383t-4b0e9f5f0622e0e7eeecedeab8a7abcba2b2fc3da772a9e836a3c64f077935253</originalsourceid><addsrcrecordid>eNp1kjtvFDEUhUcIRKKQngq5pGATv-ZVoShsyErLowBa647netYrjx3smZVS88dx2M0qKahsHZ_zXdv3FsVbRi8Y5ewSdAojDnAhNeVSiBfFKZc1XTAhxcsn-5PiPKUtpZRVDW949bo4EU3TcEbL0-LPypNfdoqBfA0enA6b4KwmNzBN92RtdxjJJ5sQEpIvoUdHlm7WtofJ-oFMGyRLY1BPJBiyGsfZZ8HvbAx-RJ9Vf0x_j2GImJLNGvieXDmHO5s5wb8pXhlwCc8P61nx82b54_p2sf72eXV9tV6AaMS0kB3F1pSGVpwjxRoRNfYIXQM1dLoD3nGjRQ91zaHFRlQgdCUNretWlLwUZ8Vqz-0DbNVdtCPEexXAqn9CiIOCOFntUEEndWe0oayspKya7oEAwpQVVnX-x8z6uGfdzd2Ivc6vjeCeQZ-feLtRQ9gpxljNaCsz4f2BEMPvGdOkRps0Ogcew5yUoFXLallSmq10b9UxpBTRHOswqh5mQT3OgjrMQo68e3q_Y-Cx89nwYW_IUbUNc8zdT__n_QWECMNc</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3069174500</pqid></control><display><type>article</type><title>In Vitro Nonalcoholic Fatty Liver Disease Model Elucidating the Effect of Immune Environment on Disease Progression and Alleviation</title><source>PubMed Central Free</source><source>American Chemical Society (ACS) Open Access</source><creator>Kim, Inhye ; Kyun, Mi-lang ; Jung, Hyewon ; Kwon, Ji-In ; Kim, Jeongha ; Kim, Ju-Kang ; Lee, Yu Bin ; Kwon, Young-In ; Moon, Kyoung-Sik</creator><creatorcontrib>Kim, Inhye ; Kyun, Mi-lang ; Jung, Hyewon ; Kwon, Ji-In ; Kim, Jeongha ; Kim, Ju-Kang ; Lee, Yu Bin ; Kwon, Young-In ; Moon, Kyoung-Sik</creatorcontrib><description>Nonalcoholic fatty liver disease (NAFLD), which is a major cause of chronic liver disease, is characterized by fat accumulation in the liver. Existing models struggle to assess medication effects on liver function in the context of NAFLD’s unique inflammatory environment. We address this by developing a 3D in vitro NAFLD model using HepG2 and THP-1 cells (mimicking liver and Kupffer cells) cocultured using transwell and hydrogel system. This mimics liver architecture and allows for manipulation of the immune environment. We demonstrate that the model recapitulates key NAFLD features: steatosis (induced by fatty acids), oxidative stress, inflammation, and impaired liver function embodying the interrelationship between NAFLD and the surrounding immune environment. This versatile model offers a valuable tool for preclinical NAFLD research by incorporating a disease-relevant immune environment.</description><identifier>ISSN: 2470-1343</identifier><identifier>EISSN: 2470-1343</identifier><identifier>DOI: 10.1021/acsomega.4c02433</identifier><identifier>PMID: 38882105</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><ispartof>ACS omega, 2024-06, Vol.9 (23), p.25094-25105</ispartof><rights>2024 The Authors. Published by American Chemical Society</rights><rights>2024 The Authors. Published by American Chemical Society.</rights><rights>2024 The Authors. Published by American Chemical Society 2024 The Authors</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-a383t-4b0e9f5f0622e0e7eeecedeab8a7abcba2b2fc3da772a9e836a3c64f077935253</cites><orcidid>0000-0002-1470-6499 ; 0000-0002-8069-8212</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acsomega.4c02433$$EPDF$$P50$$Gacs$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acsomega.4c02433$$EHTML$$P50$$Gacs$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27080,27924,27925,53791,53793,56762,56812</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38882105$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Inhye</creatorcontrib><creatorcontrib>Kyun, Mi-lang</creatorcontrib><creatorcontrib>Jung, Hyewon</creatorcontrib><creatorcontrib>Kwon, Ji-In</creatorcontrib><creatorcontrib>Kim, Jeongha</creatorcontrib><creatorcontrib>Kim, Ju-Kang</creatorcontrib><creatorcontrib>Lee, Yu Bin</creatorcontrib><creatorcontrib>Kwon, Young-In</creatorcontrib><creatorcontrib>Moon, Kyoung-Sik</creatorcontrib><title>In Vitro Nonalcoholic Fatty Liver Disease Model Elucidating the Effect of Immune Environment on Disease Progression and Alleviation</title><title>ACS omega</title><addtitle>ACS Omega</addtitle><description>Nonalcoholic fatty liver disease (NAFLD), which is a major cause of chronic liver disease, is characterized by fat accumulation in the liver. Existing models struggle to assess medication effects on liver function in the context of NAFLD’s unique inflammatory environment. We address this by developing a 3D in vitro NAFLD model using HepG2 and THP-1 cells (mimicking liver and Kupffer cells) cocultured using transwell and hydrogel system. This mimics liver architecture and allows for manipulation of the immune environment. We demonstrate that the model recapitulates key NAFLD features: steatosis (induced by fatty acids), oxidative stress, inflammation, and impaired liver function embodying the interrelationship between NAFLD and the surrounding immune environment. This versatile model offers a valuable tool for preclinical NAFLD research by incorporating a disease-relevant immune environment.</description><issn>2470-1343</issn><issn>2470-1343</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>N~.</sourceid><sourceid>DOA</sourceid><recordid>eNp1kjtvFDEUhUcIRKKQngq5pGATv-ZVoShsyErLowBa647netYrjx3smZVS88dx2M0qKahsHZ_zXdv3FsVbRi8Y5ewSdAojDnAhNeVSiBfFKZc1XTAhxcsn-5PiPKUtpZRVDW949bo4EU3TcEbL0-LPypNfdoqBfA0enA6b4KwmNzBN92RtdxjJJ5sQEpIvoUdHlm7WtofJ-oFMGyRLY1BPJBiyGsfZZ8HvbAx-RJ9Vf0x_j2GImJLNGvieXDmHO5s5wb8pXhlwCc8P61nx82b54_p2sf72eXV9tV6AaMS0kB3F1pSGVpwjxRoRNfYIXQM1dLoD3nGjRQ91zaHFRlQgdCUNretWlLwUZ8Vqz-0DbNVdtCPEexXAqn9CiIOCOFntUEEndWe0oayspKya7oEAwpQVVnX-x8z6uGfdzd2Ivc6vjeCeQZ-feLtRQ9gpxljNaCsz4f2BEMPvGdOkRps0Ogcew5yUoFXLallSmq10b9UxpBTRHOswqh5mQT3OgjrMQo68e3q_Y-Cx89nwYW_IUbUNc8zdT__n_QWECMNc</recordid><startdate>20240611</startdate><enddate>20240611</enddate><creator>Kim, Inhye</creator><creator>Kyun, Mi-lang</creator><creator>Jung, Hyewon</creator><creator>Kwon, Ji-In</creator><creator>Kim, Jeongha</creator><creator>Kim, Ju-Kang</creator><creator>Lee, Yu Bin</creator><creator>Kwon, Young-In</creator><creator>Moon, Kyoung-Sik</creator><general>American Chemical Society</general><scope>N~.</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-1470-6499</orcidid><orcidid>https://orcid.org/0000-0002-8069-8212</orcidid></search><sort><creationdate>20240611</creationdate><title>In Vitro Nonalcoholic Fatty Liver Disease Model Elucidating the Effect of Immune Environment on Disease Progression and Alleviation</title><author>Kim, Inhye ; Kyun, Mi-lang ; Jung, Hyewon ; Kwon, Ji-In ; Kim, Jeongha ; Kim, Ju-Kang ; Lee, Yu Bin ; Kwon, Young-In ; Moon, Kyoung-Sik</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a383t-4b0e9f5f0622e0e7eeecedeab8a7abcba2b2fc3da772a9e836a3c64f077935253</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Inhye</creatorcontrib><creatorcontrib>Kyun, Mi-lang</creatorcontrib><creatorcontrib>Jung, Hyewon</creatorcontrib><creatorcontrib>Kwon, Ji-In</creatorcontrib><creatorcontrib>Kim, Jeongha</creatorcontrib><creatorcontrib>Kim, Ju-Kang</creatorcontrib><creatorcontrib>Lee, Yu Bin</creatorcontrib><creatorcontrib>Kwon, Young-In</creatorcontrib><creatorcontrib>Moon, Kyoung-Sik</creatorcontrib><collection>American Chemical Society (ACS) Open Access</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals</collection><jtitle>ACS omega</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Inhye</au><au>Kyun, Mi-lang</au><au>Jung, Hyewon</au><au>Kwon, Ji-In</au><au>Kim, Jeongha</au><au>Kim, Ju-Kang</au><au>Lee, Yu Bin</au><au>Kwon, Young-In</au><au>Moon, Kyoung-Sik</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In Vitro Nonalcoholic Fatty Liver Disease Model Elucidating the Effect of Immune Environment on Disease Progression and Alleviation</atitle><jtitle>ACS omega</jtitle><addtitle>ACS Omega</addtitle><date>2024-06-11</date><risdate>2024</risdate><volume>9</volume><issue>23</issue><spage>25094</spage><epage>25105</epage><pages>25094-25105</pages><issn>2470-1343</issn><eissn>2470-1343</eissn><abstract>Nonalcoholic fatty liver disease (NAFLD), which is a major cause of chronic liver disease, is characterized by fat accumulation in the liver. Existing models struggle to assess medication effects on liver function in the context of NAFLD’s unique inflammatory environment. We address this by developing a 3D in vitro NAFLD model using HepG2 and THP-1 cells (mimicking liver and Kupffer cells) cocultured using transwell and hydrogel system. This mimics liver architecture and allows for manipulation of the immune environment. We demonstrate that the model recapitulates key NAFLD features: steatosis (induced by fatty acids), oxidative stress, inflammation, and impaired liver function embodying the interrelationship between NAFLD and the surrounding immune environment. This versatile model offers a valuable tool for preclinical NAFLD research by incorporating a disease-relevant immune environment.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>38882105</pmid><doi>10.1021/acsomega.4c02433</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0002-1470-6499</orcidid><orcidid>https://orcid.org/0000-0002-8069-8212</orcidid><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 2470-1343
ispartof ACS omega, 2024-06, Vol.9 (23), p.25094-25105
issn 2470-1343
2470-1343
language eng
recordid cdi_doaj_primary_oai_doaj_org_article_ab4cbfcf01564468b9352a3f56e67168
source PubMed Central Free; American Chemical Society (ACS) Open Access
title In Vitro Nonalcoholic Fatty Liver Disease Model Elucidating the Effect of Immune Environment on Disease Progression and Alleviation
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-26T03%3A40%3A40IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=In%20Vitro%20Nonalcoholic%20Fatty%20Liver%20Disease%20Model%20Elucidating%20the%20Effect%20of%20Immune%20Environment%20on%20Disease%20Progression%20and%20Alleviation&rft.jtitle=ACS%20omega&rft.au=Kim,%20Inhye&rft.date=2024-06-11&rft.volume=9&rft.issue=23&rft.spage=25094&rft.epage=25105&rft.pages=25094-25105&rft.issn=2470-1343&rft.eissn=2470-1343&rft_id=info:doi/10.1021/acsomega.4c02433&rft_dat=%3Cproquest_doaj_%3E3069174500%3C/proquest_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-a383t-4b0e9f5f0622e0e7eeecedeab8a7abcba2b2fc3da772a9e836a3c64f077935253%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=3069174500&rft_id=info:pmid/38882105&rfr_iscdi=true