Genomic Insights of First ermB-Positive ST338-SCCmecVT/CC59 Taiwan Clone of Community-Associated Methicillin-Resistant Staphylococcus aureus in Poland

• Published in: International journal of molecular sciences 2022-08, Vol.23 (15), p.8755
• Main Authors: Szymanek-Majchrzak, Ksenia, Młynarczyk, Grażyna
• Format: Article
• Language: English
• Subjects: Aminoglycoside antibiotics; Aminoglycosides; Antibiotics; Antimicrobial agents; CA-MRSA; Chromosomes; Cloning; Drug resistance; Epidemiology; ErmB gene; Genes; Genomes; Genomics; Genotype & phenotype; Insertion sequences; IS1216V insertion sequence; Lincosamides; macrolides lincosamides and streptogramin B (MLS-B); Methicillin; mobile element structure (MES PM1); Nosocomial infection; Open reading frames; Panton–Valentine leukocidin (PVL); Peptides; Protein sorting signals; Proteins; Staphylococcus aureus; Staphylococcus infections; Streptogramin B; Streptothricin; β-Lactam antibiotics
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms23158755

We report the first Polish representative of community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA), lukS/F-PV-positive, encoding the ermB gene, as a genetic determinant of constitutive resistance to macrolides, lincosamides, and streptogramin B antibiotics, cMLS-B. This is the first detection of the CA-MRSA strain responsible for nosocomial infection in the Warsaw Clinical Hospital. Resistance to β-lactams associates with a composite genetic element, SCCmec cassette type VT (5C2&5). We assigned the strain to sequence type ST338 (single-locus variant of ST59), clonal complex CC59, spa-type t437, and agr-type I. Genomic-based comparison was designated SO574/12 as an international Taiwan clone, which has been so far described mainly in the Asia-Pacific region. The ermB gene locates on the chromosome within the 14,690 bp mobile element structure, i.e., the MESPM1-like structure, which also encodes aminoglycoside- and streptothricin-resistance genes. The MESPM1-like structure is a composite transposon containing Tn551, flanked by direct repeats of IS1216V insertion sequences, which probably originates from Enterococcus. The ermB is preceded by the 273 bp regulatory region that contains the regulatory 84 bp ermBL ORF, encoding the 27 amino acid leader peptides. The latest research suggests that a new leader peptide, ermBL2, also exists in the ermB regulatory region. Therefore, the detailed function of ermBL2 requires further investigations.