Characterisation of Canine and Feline Breast Tumours, Their Metastases, and Corresponding Primary Cell Lines Using LA-REIMS and DESI-MS Imaging

Breast cancer, a complex disease with a significant prevalence to form metastases, necessitates novel therapeutic strategies to improve treatment outcomes. Here, we present the results of a comparative molecular study of primary breast tumours, their metastases, and the corresponding primary cell li...

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Bibliographic Details
Published in:International journal of molecular sciences 2024-07, Vol.25 (14), p.7752
Main Authors: Molnár, Adrienn, Horkovics-Kováts, Gabriel Stefan, Kucsma, Nóra, Szegő, Zsuzsanna, Tauber, Boglárka, Egri, Attila, Szkupien, Zoltán, Deák, Bálint András, McKenzie, James S, Thuróczy, Julianna, Schäffer, Richard, Schlosser, Gitta, Szakács, Gergely, Balog, Júlia
Format: Article
Language:English
Subjects:
Algorithms
ambient ionisation
Annotations
Breast cancer
Cancer research
Cancer therapies
Chemotherapy
Lasers
lipidomics
Lipids
Mass spectrometry
mass spectrometry imaging
Medical research
metabolic fingerprints
Metastasis
primary cell lines
Scientific imaging
Tumors
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Summary:Breast cancer, a complex disease with a significant prevalence to form metastases, necessitates novel therapeutic strategies to improve treatment outcomes. Here, we present the results of a comparative molecular study of primary breast tumours, their metastases, and the corresponding primary cell lines using Desorption Electrospray Ionisation (DESI) and Laser-Assisted Rapid Evaporative Ionisation Mass Spectrometry (LA-REIMS) imaging. Our results show that ambient ionisation mass spectrometry technology is suitable for rapid characterisation of samples, providing a lipid- and metabolite-rich spectrum within seconds. Our study demonstrates that the lipidomic fingerprint of the primary tumour is not significantly distinguishable from that of its metastasis, in parallel with the similarity observed between their respective primary cell lines. While significant differences were observed between tumours and the corresponding cell lines, distinct lipidomic signatures and several phospholipids such as PA(36:2), PE(36:1), and PE(P-38:4)/PE(O-38:5) for LA-REIMS imaging and PE(P-38:4)/PE(O-38:5), PS(36:1), and PI(38:4) for DESI-MSI were identified in both tumours and cells. We show that the tumours' characteristics can be found in the corresponding primary cell lines, offering a promising avenue for assessing tumour responsiveness to therapeutic interventions. A comparative analysis by DESI-MSI and LA-REIMS imaging revealed complementary information, demonstrating the utility of LA-REIMS in the molecular imaging of cancer.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms25147752