Enzymatic synthesis of new antimicrobial peptides for food purposes

• Published in: Frontiers in microbiology 2023-05, Vol.14, p.1153135-1153135
• Main Authors: Adaro, Mauricio, Ibáñez, Ángel Gabriel Salinas, Origone, Anabella Lucia, Vallés, Diego, Guzmán, Fanny, Vega, Alba, Barberis, Sonia
• Format: Article
• Language: English
• Subjects: antibacterial peptide; enzymatic synthesis of peptides; food preservation; Ile-Gln (IQ); Microbiology; novel peptide against sensitive and SDR Gram positive and Gram negative strains
• ISSN: 1664-302X; 1664-302X
• DOI: 10.3389/fmicb.2023.1153135

Growing consumer awareness of the potential negative health effects of synthetic antibiotics has prompted the search for more natural preservatives that can improve the safety and quality of food. In this study we report the enzymatic synthesis of N-α-[Carbobenzyloxy]-Ile-Gln (Z-IQ) which is the precursor of Ile-Gln (IQ), a new antibacterial dipeptide, using an aqueous-organic biphasic system formed by 50% (v/v) ethyl acetate in 0.1 M Tris - HCl buffer pH 8. A partially purified proteolytic extract from the fruits of named granulosain, proved to be a robust biocatalyst for the synthesis of Z-IQ, eliciting 71 ± 0.10% maximal peptide yield in the above described conditions. After cleaving and purifying IQ dipeptide, antimicrobial activity was assayed against ATCC 25923, A17771, and C00195, and MIC values between 118 ± 0.01 μg/mL and 133.7 ± 0.05 μg/mL were obtained. In addition, IQ showed MIC of 82.4 ± 0.01 μg/mL and 85.0 ± 0.00 μg/mL against ATCC 25922 and A17683, respectively. IQ did not show inhibitory activity against single-drug resistance (SDR) strains, such as A19438 (SDR) and C00213 (SDR), and against multidrug-resistant I00125 (MDR). IQ also caused growth inhibition of NCTC 11638 and three wild-type strains, which are sensitive to AML, MTZ, LEV and CLA ( 659), resistant to LEV ( 661 SDR), and resistant to MTZ ( 662 SDR). Finally, this study contributes with a new dipeptide (IQ) that can be used as an antimicrobial agent for food preservation or as a safe ingredient of functional foods.