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Demixing fluorescence time traces transmitted by multimode fibers
Optical methods based on thin multimode fibers (MMFs) are promising tools for measuring neuronal activity in deep brain regions of freely moving mice thanks to their small diameter. However, current methods are limited: while fiber photometry provides only ensemble activity, imaging techniques using...
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| Published in: | Nature communications 2024-07, Vol.15 (1), p.6286-14, Article 6286 |
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| creator | Rimoli, Caio Vaz Moretti, Claudio Soldevila, Fernando Brémont, Enora Ventalon, Cathie Gigan, Sylvain |
| description | Optical methods based on thin multimode fibers (MMFs) are promising tools for measuring neuronal activity in deep brain regions of freely moving mice thanks to their small diameter. However, current methods are limited: while fiber photometry provides only ensemble activity, imaging techniques using of long multimode fibers are very sensitive to bending and have not been applied to unrestrained rodents yet. Here, we demonstrate the fundamentals of a new approach using a short MMF coupled to a miniscope. In proof-of-principle in vitro experiments, we disentangled spatio-temporal fluorescence signals from multiple fluorescent sources transmitted by a thin (200 µm) and short (8 mm) MMF, using a general unconstrained non-negative matrix factorization algorithm directly on the raw video data. Furthermore, we show that low-cost open-source miniscopes have sufficient sensitivity to image the same fluorescence patterns seen in our proof-of-principle experiment, suggesting a new avenue for novel minimally invasive deep brain studies using multimode fibers in freely behaving mice.
The Authors demonstrate how time traces of individual fluorescent sources can be demixed from spatio-temporal intensity patterns transmitted by short multimode fibers. This is a first step towards measuring activity of single sources in fiber photometry experiments. |
| doi_str_mv | 10.1038/s41467-024-50306-z |
| format | article |
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The Authors demonstrate how time traces of individual fluorescent sources can be demixed from spatio-temporal intensity patterns transmitted by short multimode fibers. 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| subjects | 639/624/1075/187 639/624/1107/328/2235 639/624/1107/510 Algorithms Brain Calibration Demixing Experiments Fibers Fluorescence Humanities and Social Sciences Imaging techniques Light multidisciplinary Neuroimaging Neurons Neurosciences Optics Photometry Physics Science Science (multidisciplinary) Time measurement Video data |
| title | Demixing fluorescence time traces transmitted by multimode fibers |
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