Anti-leishmanial activity of Avicennia marina (Avicenniaceae family) leaves hydroalcoholic extract and its possible cellular mechanisms

• Published in: Parasite epidemiology and control 2022-05, Vol.17, p.e00239-e00239, Article e00239
• Main Authors: Rashno, Zahra, Sharifi, Iraj, Oliaee, Razieh Tavakoli, Tajbakhsh, Saeed, Sharififar, Fariba, Sharifi, Fatemeh, Hatami, Abbas, Faridi, Ashkan, Babaei, Zahra
• Format: Article
• Language: English
• Subjects: Arginase activity; Avicennia marina; Leishmania tropica; MTT; Original Research article; ROS
• ISSN: 2405-6731; 2405-6731
• DOI: 10.1016/j.parepi.2022.e00239

Natural products are the main source of potent antioxidants and anti-leishmanial agents. This study was aimed to evaluate Avicennia marina (Avicenniaceae family) extract inhibitory effect against Leishmania tropica by accessing apoptotic markers and arginase activity. The A. marina were extracted and phytochemical analysis conducted. The inhibitory effect of A. marina was evaluated on L. tropica promastigote and amastigote forms, compared to meglumine antimoniate (Glucantime, MA) as standard drug. The level of apoptosis, Reactive Oxygen Species (ROS) production and arginase activity was assessed in A. marina-treated cells compared to control group. Phytochemical screening of A. marina extract showed strong presence of tannins and saponins. We demonstrated the inhibitory effect of A. marina on promastigote stages in a dose dependent manner. Also, lower 50% inhibitory concentration (IC50) value of amastigotes was indicated in A. marina group compared with the standard group of Glucantime (60.57 ± 1.46 vs. 73.19 ± 10.12 μg/mL, respectively, P