Putative alternative translation start site-encoding nucleotides of CPR5 regulate growth and resistance

• Published in: BMC plant biology 2020-06, Vol.20 (1), p.295-10, Article 295
• Main Authors: Faisal, Muhammad B, Gechev, Tsanko S, Mueller-Roeber, Bernd, Dijkwel, Paul P
• Format: Article
• Language: English
• Subjects: Arabidopsis; Arabidopsis - genetics; Arabidopsis - microbiology; Arabidopsis Proteins - genetics; Arabidopsis Proteins - physiology; Arabidopsis thaliana; Cell death; Complementation; Computer Simulation; CPR5; Disease resistance; Disease Resistance - genetics; Endoreduplication; Gating; Genes; Glutamine; Growth; Kinases; Levels; Localization; Membrane Proteins - genetics; Membrane Proteins - physiology; Methionine; Microbial drug resistance; Mutagenesis; Mutation; N-Terminus; Nucleotides; Nucleotides - genetics; Pathogenesis; Peptide Chain Initiation, Translational; Phenotypes; Plant diseases; Plant Diseases - genetics; Plant Diseases - microbiology; Plant growth; Plant Leaves; Plants, Genetically Modified; Ploidies; Ploidy; Promoter Regions, Genetic; Proteins; Pseudomonas syringae; Senescence; Translation; Transmembrane domains; Trichomes
• ISSN: 1471-2229; 1471-2229
• DOI: 10.1186/s12870-020-02485-2

The Arabidopsis CONSTITUTIVE EXPRESSER of PATHOGENESIS-RELATED GENES 5 (CPR5) has recently been shown to play a role in gating as part of the nuclear pore complex (NPC). Mutations in CPR5 cause multiple defects, including aberrant trichomes, reduced ploidy levels, reduced growth and enhanced resistance to bacterial and fungal pathogens. The pleiotropic nature of cpr5 mutations implicates that the CPR5 protein affects multiple pathways. However, little is known about the structural features that allow CPR5 to affect the different pathways. Our in silico studies suggest that in addition to three clusters of putative nuclear localization signals and four or five transmembrane domains, CPR5 contains two putative alternative translation start sites. To test the role of the methionine-encoding nucleotides implicated in those sites, metCPR5 cDNAs, in which the relevant nucleotides were changed to encode glutamine, were fused to the CPR5 native promoter and the constructs transformed to cpr5-2 plants to complement cpr5-compromised phenotypes. The control and metCPR5 constructs were able to complement all cpr5 phenotypes, although the extent of complementation depended on the specific complementing plant lines. Remarkably, plants transformed with metCPR5 constructs showed larger leaves and displayed reduced resistance when challenged to Pseudomonas syringae pv Pst DC3000, as compared to control plants. Thus, the methionine-encoding nucleotides regulate growth and resistance. We propose that structural features of the CPR5 N-terminus are implicated in selective gating of proteins involved in regulating the balance between growth and resistance. Plants need to carefully balance the amount of resources used for growth and resistance. The Arabidopsis CPR5 protein regulates plant growth and immunity. Here we show that N-terminal features of CPR5 are involved in the regulation of the balance between growth and resistance. These findings may benefit efforts to improve plant yield, while maintaining optimal levels of disease resistance.