Bioinspired detoxification of blood: The efficient removal of anthrax toxin protective antigen using an extracorporeal macroporous adsorbent device

Whilst various remedial human monoclonal antibodies have been developed to treat the potentially life-threatening systemic complications associated with anthrax infection, an optimal and universally effective administration route has yet to be established. In the later stages of infection when antib...

Full description

Saved in:
Bibliographic Details
Published in:Scientific reports 2018-05, Vol.8 (1), p.7518-11, Article 7518
Main Authors: Ingavle, Ganesh, Baillie, Les, Davies, Nathan, Beaton, Nigel, Zheng, Yishan, Mikhalovsky, Sergey, Sandeman, Susan
Format: Article
Language:English
Subjects:
14/19
14/28
631/61/54/990
692/699/255/1318
82/1
Anthrax
Antigens
Blood levels
Detoxification
Exotoxins
Humanities and Social Sciences
Monoclonal antibodies
multidisciplinary
Polymers
Protective antigen
Science
Science (multidisciplinary)
Toxins
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Whilst various remedial human monoclonal antibodies have been developed to treat the potentially life-threatening systemic complications associated with anthrax infection, an optimal and universally effective administration route has yet to be established. In the later stages of infection when antibody administration by injection is more likely to fail one possible route to improve outcome is via the use of an antibody-bound, adsorbent haemoperfusion device. We report here the development of an adsorbent macroporous polymer column containing immobilised B . anthracis exotoxin-specific antibodies, PANG (a non-glycosylated, version of a plant-produced human monoclonal antibody) and Valortim (a fully human monoclonal N-linked glycosylated antibody), for removal of anthrax protective antigen (PA) from freshly frozen human plasma and human whole blood. In addition, we have demonstrated that continuous extracorporeal blood recirculation through a Valortim-bound haemoperfusion column significantly reduced the blood plasma concentration of anthrax PA over 2 hours using an in vivo PA rat infusion model. This work provides proof-of-concept evidence to support the development of such alternative detoxification platforms.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-018-25678-0