Macrophage inhibitory cytokine‐1 induced by a high‐fat diet promotes prostate cancer progression by stimulating tumor‐promoting cytokine production from tumor stromal cells

• Published in: Cancer communications (London, England) England), 2021-05, Vol.41 (5), p.389-403
• Main Authors: Huang, Mingguo, Narita, Shintaro, Koizumi, Atsushi, Nara, Taketoshi, Numakura, Kazuyuki, Satoh, Shigeru, Nanjo, Hiroshi, Habuchi, Tomonori
• Format: Article
• Language: English
• Subjects: Adipocytes; Carbohydrates; Cell growth; Cytokines; Diet; Fibroblasts; Gene expression; Glycerol; high‐fat diet; Kinases; macrophage inhibitory cytokine‐1; metabolism; Obesity; Oils & fats; Original; Prostate cancer; Smooth muscle; tumor microenvironment; Tumorigenesis
• ISSN: 2523-3548; 2523-3548
• DOI: 10.1002/cac2.12137

Background Recent studies have indicated that a high‐fat diet (HFD) and/or HFD‐induced obesity may influence prostate cancer (PCa) progression, but the role of HFD in PCa microenvironment is unclear. This study aimed to delineate the molecular mechanisms of PCa progression under HFD milieus and define the stromal microenvironment focusing on macrophage inhibitory cytokine‐1 (MIC‐1) activation. Methods We investigated the effects of HFD on PCa stromal microenvironment and MIC‐1 signaling activation using PC‐3M‐luc‐C6 PCa model mice fed with HFD or control diet. Further, we explored the effect of periprostatic adipocytes derived from primary PCa patients on activation and cytokine secretion of prostate stromal fibroblasts. Expression patterns and roles of MIC‐1 signaling on human PCa stroma activation and progression were also investigated. Results HFD stimulated PCa cell growth and invasion as a result of upregulated MIC‐1 signaling and subsequently increased the secretion of interleukin (IL)‐8 and IL‐6 from prostate stromal fibroblasts in PC‐3M‐luc‐C6 PCa mouse model. In addition, periprostatic adipocytes directly stimulated MIC‐1 production from PC‐3 cells and IL‐8 secretion in prostate stromal fibroblasts through the upregulation of adipose lipolysis and free fatty acid release. The increased serum MIC‐1 was significantly correlated with human PCa stroma activation, high serum IL‐8, IL‐6, and lipase activity, advanced PCa progression, and high body mass index of the patients. Glial‐derived neurotrophic factor receptor α‐like (GFRAL), a specific receptor of MIC‐1, was highly expressed in both cytoplasm and membrane of PCa cells and surrounding stromal fibroblasts, and the expression level was decreased by androgen deprivation therapy and chemotherapy. Conclusion HFD‐mediated activation of the PCa stromal microenvironment through metabolically upregulated MIC‐1 signaling by increased available free fatty acids may be a critical mechanism of HFD and/or obesity‐induced PCa progression. MIC‐1 production was increased in PCa cells, which was affected by adipocyte infiltration and adipolysis. MIC‐1 directly stimulated the surrounding PrSC cells to secrete protumorigenic cytokines such as IL‐8 and IL‐6 in the PCa stromal microenvironment, especially under a HFD condition. These upregulated functional cytokines directly and/or indirectly stimulated PCa cell proliferation, invasion, and metastasis.