The flavonoid hesperidin exerts anti-photoaging effect by downregulating matrix metalloproteinase (MMP)-9 expression via mitogen activated protein kinase (MAPK)-dependent signaling pathways

• Published in: BMC complementary and alternative medicine 2018-01, Vol.18 (1), p.39-39, Article 39
• Main Authors: Lee, Hee Jeong, Im, A-Rang, Kim, Su-Man, Kang, Hyung-Sik, Lee, Jae Dong, Chae, Sungwook
• Format: Article
• Language: English
• Subjects: Aging; Animals; Antioxidants; Antioxidants - pharmacology; Apoptosis; Bioflavonoids; Body weight; Cell adhesion & migration; Chronic exposure; Collagen; Cytokines; Down-Regulation - drug effects; Epidermis - drug effects; Epidermis - radiation effects; Extracellular matrix; Flavones; Flavonoids; Fuel consumption; Gelatinase B; Gene expression; Hairless; Health aspects; Hesperidin; Hesperidin - pharmacology; Hypertrophy; Immunohistochemistry; Inflammation; Irradiation; Kinases; Male; MAP kinase; MAP Kinase Signaling System - drug effects; Matrix metalloproteinase; Matrix Metalloproteinase 9 - metabolism; Matrix metalloproteinase-9; Metalloproteinase; Mice; Mice, Hairless; Oral administration; Permeability; Phosphotransferases; Photoaging; Protein kinase; Proteins; Rodents; Signal transduction; Signaling; Skin; Skin Aging - drug effects; Skin Aging - radiation effects; Transepidermal water loss; Tumor necrosis factor-TNF; Ultraviolet B; Ultraviolet radiation; Ultraviolet Rays; Water loss
• ISSN: 1472-6882; 1472-6882
• DOI: 10.1186/s12906-017-2058-8

Hesperidin is a flavonoid with antioxidant, anti-inflammatory, and immune modulatory activities. Photoaging is a consequence of chronic exposure to the sun and ultraviolet (UV) radiation. This study was designed to evaluate the efficacy of hesperidin against photoaging of dorsal skin in hairless mice. Hairless male mice (6-week-old) were divided into three groups (n = 7): control, UVB-treated vehicle, and UVB-treated hesperidin groups. UVB-irradiated mice from hesperidin group were orally administered 0.1 mL of water containing 100 mg/kg body weight per day hesperidin. The mean length and depth of wrinkles in the UVB-treated hesperidin group significantly improved after the oral administration of hesperidin, which significantly inhibited the increase in epidermal thickness and epidermal hypertrophy (P < 0.05). UVB irradiation of mice induced epidermal barrier dysfunction including an increase in the transepidermal water loss (TEWL); however, hesperidin decreased the TEWL. UVB irradiation increased the expression of MMP-9 and pro-inflammatory cytokines whereas UVB-treated hesperidin group showed reduced expression. These results indicate that hesperidin showed anti-photoaging activity in the UVB-irradiated hairless mice. In conclusion, hesperidin inhibited the UVB-induced increase in skin thickness, wrinkle formation, and collagen fiber loss in male hairless mice. These results suggest that hesperidin shows potent anti-photoaging activity by regulating MMP-9 expression through the suppression of MAPK-dependent signaling pathways.