Circadian RNA expression elicited by 3'-UTR IRAlu-paraspeckle associated elements

Paraspeckles are nuclear bodies form around the long non-coding RNA, Neat1, and RNA-binding proteins. While their role is not fully understood, they are believed to control gene expression at a post-transcriptional level by means of the nuclear retention of mRNA containing in their 3'-UTR inver...

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Bibliographic Details
Published in:eLife 2016-07, Vol.5
Main Authors: Torres, Manon, Becquet, Denis, Blanchard, Marie-Pierre, Guillen, Séverine, Boyer, Bénédicte, Moreno, Mathias, Franc, Jean-Louis, François-Bellan, Anne-Marie
Format: Article
Language:English
Subjects:
3' Untranslated Regions
Animals
Antisense RNA
Cell Biology
Cell Line
circadian oscillators
Circadian Rhythm
Circadian rhythms
Gene expression
Gene Expression Regulation
Genes and Chromosomes
Genes, Reporter
Genetic aspects
Genomes
Green Fluorescent Proteins - analysis
Green Fluorescent Proteins - genetics
Immunoglobulins
Intravital Microscopy
Inverted Repeat Sequences
Life Sciences
Methods
Microscopy
Microscopy, Video
Neurobiology
Neurons and Cognition
Non-coding RNA
Nuclear Proteins - biosynthesis
Nuclear Proteins - genetics
nuclear RNA retention
Observations
paraspeckle
Pituitary
pituitary cells
Post-transcription
Proteins
Rats
RNA sequencing
RNA, Long Noncoding - biosynthesis
RNA, Long Noncoding - genetics
RNA-binding protein
RNA-Binding Proteins - biosynthesis
RNA-Binding Proteins - genetics
Software
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Summary:Paraspeckles are nuclear bodies form around the long non-coding RNA, Neat1, and RNA-binding proteins. While their role is not fully understood, they are believed to control gene expression at a post-transcriptional level by means of the nuclear retention of mRNA containing in their 3'-UTR inverted repeats of Alu sequences (IRAlu). In this study, we found that, in pituitary cells, all components of paraspeckles including four major proteins and Neat1 displayed a circadian expression pattern. Furthermore the insertion of IRAlu at the 3'-UTR of the EGFP cDNA led to a rhythmic circadian nuclear retention of the egfp mRNA that was lost when paraspeckles were disrupted whereas insertion of a single antisense Alu had only a weak effect. Using real-time video-microscopy, these IRAlu were further shown to drive a circadian expression of EGFP protein. This study shows that paraspeckles, thanks to their circadian expression, control circadian gene expression at a post-transcriptional level.
ISSN:2050-084X
2050-084X
DOI:10.7554/elife.14837