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Claudin-1 Expression Is Elevated in Colorectal Cancer Precursor Lesions Harboring the BRAF V600E Mutation

Abstract BACKGROUND : Sessile serrated adenomas/polyps (SSA/P) are now recognised precursors of colorectal cancer (CRC) including cancers harbouring somatic BRAF (V600E) mutations. While the morphological diagnostic criteria of SSA/P have been established, distinguishing between small/early SSA/P an...

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Published in:Translational oncology 2014-08, Vol.7 (4), p.456-463
Main Authors: Caruso, Maria, Fung, Kim Y.C, Moore, James, Brierley, Gemma V, Cosgrove, Leah J, Thomas, Michelle, Cheetham, Glenice, Brook, Emma, Fraser, Louise M, Tin, Teresa, Tran, Ha, Ruszkiewicz, Andrew
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Language:English
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Summary:Abstract BACKGROUND : Sessile serrated adenomas/polyps (SSA/P) are now recognised precursors of colorectal cancer (CRC) including cancers harbouring somatic BRAF (V600E) mutations. While the morphological diagnostic criteria of SSA/P have been established, distinguishing between small/early SSA/P and microvesicular hyperplastic polyps (MVHP) is challenging and may not be possible in routine practice. METHODS : Gene expression profiling of MVHP ( n =5, all BRAF V600E wild-type ) and SSA/P ( n =5, all BRAF V600E mutant) samples was performed. Quantitative reverse transcription–polymerase chain reaction (qRT-PCR) and immunohistochemical analysis was performed to verify the expression of claudin 1 (CLDN1) in MVHP and SSA/P. RESULTS : Gene expression profiling studies conducted between MVHP and SSA/P identified CLDN1 as the most statistically significant differentially expressed gene ( p
ISSN:1936-5233
1944-7124
DOI:10.1016/j.tranon.2014.05.009