Circulating miR-30a-5p and miR-182-5p in Prediabetes and Screen-Detected Diabetes Mellitus

• Published in: Diabetes, metabolic syndrome and obesity metabolic syndrome and obesity, 2020-01, Vol.13, p.5037-5047
• Main Authors: Weale, Cecil Jack, Matshazi, Don M, Davids, Saarah F G, Raghubeer, Shanel, Erasmus, Rajiv T, Kengne, Andre Pascal, Davison, Glenda Mary, Matsha, Tandi E
• Format: Article
• Language: English
• Subjects: africa; Age; Analysis; Blood cholesterol; Blood pressure; Body mass index; Cholesterol; Dextrose; Diabetes; Ethics; Fasting; Glucose; Glycosylated hemoglobin; Insulin; Investigations; Low density lipoprotein; Low density lipoproteins; Medical screening; Metabolism; MicroRNA; MicroRNAs; mir-182-5p; mir-30a-5p; Original Research; prediabetes; Prediabetic state; Regression analysis; Standard deviation; Type 2 diabetes; Womens health
• ISSN: 1178-7007; 1178-7007
• DOI: 10.2147/DMSO.S286081

microRNAs (miRNAs) have been touted as potential diagnostic and prognostic biomarkers for various diseases. The aim of the present study was to evaluate the diagnostic value of miR-30a-5p and miR-182-5p for prediabetes and screen-detected type 2 diabetes mellitus (T2DM). The study included 1270 participants (207 prediabetes, 94 screen-detected diabetes and 969 normotolerant) from the Vascular and Metabolic Health (VMH) study. Whole blood levels of miR-30a-5p and miR-182-5p were quantitated by RT-qPCR. Multivariable logistic regressions were used to relate miRNAs with prediabetes or T2DM and receiver operating characteristic (ROC) curves were used to evaluate the ability of each miRNA to diagnose these conditions. Both miRNAs were significantly highly expressed in individuals with prediabetes or T2DM (both ≥3.2-fold, and