Use of lymphocyte cultures for BrdU replication banding patterns in anuran species (Amphibia)

We describe the standardization of lymphocyte culture procedures in order to improve cytological preparations of anuran species. This methodology permits the use of 5-bromodeoxyuridine (BrdU) treatment to obtain replication banding patterns in the chromosomes of these species. Descreve-se a padroniz...

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Bibliographic Details
Published in:Genetics and molecular biology 1998-12, Vol.21 (4), p.471-476
Main Authors: Kasahara, Sanae, Silva, Ana Paula Zampieri, Gruber, Simone Lilian
Format: Article
Language:English
Subjects:
BIOCHEMISTRY & MOLECULAR BIOLOGY
GENETICS & HEREDITY
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Summary:We describe the standardization of lymphocyte culture procedures in order to improve cytological preparations of anuran species. This methodology permits the use of 5-bromodeoxyuridine (BrdU) treatment to obtain replication banding patterns in the chromosomes of these species. Descreve-se a padronização da cultura de linfócitos com a finalidade de melhorar as preparações citológicas de espécies de anfíbios anuros. Esta metodologia permite o uso do tratamento pelo BrdU para se obter padrões de bandas de replicação nos cromossomos dessas espécies.
ISSN:1415-4757
1678-4685
1415-4757
1678-4685
DOI:10.1590/S1415-47571998000400011