Single Cell and Single Nucleus RNA-Seq Reveal Cellular Heterogeneity and Homeostatic Regulatory Networks in Adult Mouse Stria Vascularis

The stria vascularis (SV) generates the endocochlear potential (EP) in the inner ear and is necessary for proper hair cell mechanotransduction and hearing. While channels belonging to SV cell types are known to play crucial roles in EP generation, relatively little is known about gene regulatory net...

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Published in:Frontiers in molecular neuroscience 2019-12, Vol.12, p.316-316
Main Authors: Korrapati, Soumya, Taukulis, Ian, Olszewski, Rafal, Pyle, Madeline, Gu, Shoujun, Singh, Riya, Griffiths, Carla, Martin, Daniel, Boger, Erich, Morell, Robert J, Hoa, Michael
Format: Article
Language:English
Subjects:
adult stria vascularis
Deafness
Ears & hearing
Genomics
hearing
Hearing loss
Inner ear
Life sciences
Mechanotransduction
Molecular modelling
Mutation
Neuroscience
Potassium
regulons
Ribonucleic acid
RNA
scRNA-Seq
snRNA-Seq
Stria vascularis
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Summary:The stria vascularis (SV) generates the endocochlear potential (EP) in the inner ear and is necessary for proper hair cell mechanotransduction and hearing. While channels belonging to SV cell types are known to play crucial roles in EP generation, relatively little is known about gene regulatory networks that underlie the ability of the SV to generate and maintain the EP. Using single cell and single nucleus RNA-sequencing, we identify and validate known and rare cell populations in the SV. Furthermore, we establish a basis for understanding molecular mechanisms underlying SV function by identifying potential gene regulatory networks as well as druggable gene targets. Finally, we associate known deafness genes with adult SV cell types. This work establishes a basis for dissecting the genetic mechanisms underlying the role of the SV in hearing and will serve as a basis for designing therapeutic approaches to hearing loss related to SV dysfunction.
ISSN:1662-5099
1662-5099
DOI:10.3389/fnmol.2019.00316