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Hydroxytyrosol Modulates Adipocyte Gene and miRNA Expression Under Inflammatory Condition

Chronic inflammation of the adipose tissue (AT) is a major contributor to obesity-associated cardiometabolic complications. The olive oil polyphenol hydroxytyrosol (HT) contributes to Mediterranean diet cardiometabolic benefits through mechanisms still partially unknown. We investigated HT (1 and 10...

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Published in:Nutrients 2019-10, Vol.11 (10), p.2493
Main Authors: Scoditti, Egeria, Carpi, Sara, Massaro, Marika, Pellegrino, Mariangela, Polini, Beatrice, Carluccio, Maria Annunziata, Wabitsch, Martin, Verri, Tiziano, Nieri, Paola, De Caterina, Raffaele
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cited_by cdi_FETCH-LOGICAL-c472t-6b48303e60c567bb07b159583ea3639b7d1ae71b8fde87d3a6cf015be19abf503
cites cdi_FETCH-LOGICAL-c472t-6b48303e60c567bb07b159583ea3639b7d1ae71b8fde87d3a6cf015be19abf503
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container_issue 10
container_start_page 2493
container_title Nutrients
container_volume 11
creator Scoditti, Egeria
Carpi, Sara
Massaro, Marika
Pellegrino, Mariangela
Polini, Beatrice
Carluccio, Maria Annunziata
Wabitsch, Martin
Verri, Tiziano
Nieri, Paola
De Caterina, Raffaele
description Chronic inflammation of the adipose tissue (AT) is a major contributor to obesity-associated cardiometabolic complications. The olive oil polyphenol hydroxytyrosol (HT) contributes to Mediterranean diet cardiometabolic benefits through mechanisms still partially unknown. We investigated HT (1 and 10 μmol/L) effects on gene expression (mRNA and microRNA) related to inflammation induced by 10 ng/mL tumor necrosis factor (TNF)-α in human Simpson-Golabi-Behmel Syndrome (SGBS) adipocytes. At real-time PCR, HT significantly inhibited TNF-α-induced mRNA levels, of monocyte chemoattractant protein-1, C-X-C Motif Ligand-10, interleukin (IL)-1β, IL-6, vascular endothelial growth factor, plasminogen activator inhibitor-1, cyclooxygenase-2, macrophage colony-stimulating factor, matrix metalloproteinase-2, Cu/Zn superoxide dismutase-1, and glutathione peroxidase, as well as surface expression of intercellular adhesion molecule-1, and reverted the TNF-α-mediated inhibition of endothelial nitric oxide synthase, peroxisome proliferator-activated receptor coactivator-1α, and glucose transporter-4. We found similar effects in adipocytes stimulated by macrophage-conditioned media. Accordingly, HT significantly counteracted miR-155-5p, miR-34a-5p, and let-7c-5p expression in both cells and exosomes, and prevented NF-κB activation and production of reactive oxygen species. HT can therefore modulate adipocyte gene expression profile through mechanisms involving a reduction of oxidative stress and NF-κB inhibition. By such mechanisms, HT may blunt macrophage recruitment and improve AT inflammation, preventing the deregulation of pathways involved in obesity-related diseases.
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The olive oil polyphenol hydroxytyrosol (HT) contributes to Mediterranean diet cardiometabolic benefits through mechanisms still partially unknown. We investigated HT (1 and 10 μmol/L) effects on gene expression (mRNA and microRNA) related to inflammation induced by 10 ng/mL tumor necrosis factor (TNF)-α in human Simpson-Golabi-Behmel Syndrome (SGBS) adipocytes. At real-time PCR, HT significantly inhibited TNF-α-induced mRNA levels, of monocyte chemoattractant protein-1, C-X-C Motif Ligand-10, interleukin (IL)-1β, IL-6, vascular endothelial growth factor, plasminogen activator inhibitor-1, cyclooxygenase-2, macrophage colony-stimulating factor, matrix metalloproteinase-2, Cu/Zn superoxide dismutase-1, and glutathione peroxidase, as well as surface expression of intercellular adhesion molecule-1, and reverted the TNF-α-mediated inhibition of endothelial nitric oxide synthase, peroxisome proliferator-activated receptor coactivator-1α, and glucose transporter-4. 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subjects adipocyte
Adipocytes
Adipocytes - drug effects
Adipocytes - metabolism
Adipogenesis
Angiogenesis
Antioxidants
Cell activation
Cell Line
Chemokines
Cyclooxygenase-2
Diabetes
Diet
DNA
Endocrinology
Endothelial cells
Ethanol
exosome
Exosomes - metabolism
extra virgin olive oil
Extracellular matrix
Gelatinase A
Gelatinase B
Gene expression
Gene Expression Regulation - drug effects
Gene regulation
Growth factors
High fat diet
Humans
hydroxytyrosol
Inflammation
Inflammation - chemically induced
Inflammation - metabolism
Insulin
insulin resistance
Intercellular adhesion molecule 1
Interleukin 6
Lipids
Maintenance
Matrix metalloproteinases
Metabolic syndrome
Metabolites
MicroRNAs
MicroRNAs - genetics
MicroRNAs - metabolism
miRNA
Obesity
Oils & fats
Olive oil
Oxidative stress
Pediatrics
Phenylethyl Alcohol - analogs & derivatives
Phenylethyl Alcohol - pharmacology
Physiology
polyphenol
Polyphenols
Protein Binding
Reactive Oxygen Species
Scavenging
Transcription Factor RelA - metabolism
Tumor Necrosis Factor-alpha - pharmacology
Tumor necrosis factor-TNF
Tumor necrosis factor-α
title Hydroxytyrosol Modulates Adipocyte Gene and miRNA Expression Under Inflammatory Condition
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