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Emergence of fluoroquinolone resistance and possible mechanisms in clinical isolates of Stenotrophomonas maltophilia from Iran
Stenotrophomonas maltophilia exhibits wide spectrum of fluoroquinolone resistance using different mechanisms as multidrug efflux pumps and Sm qnr alleles. Here, the role of smeDEF , smeVWX efflux genes and contribution of Sm qnr alleles in the development of fluoroquinolone resistance was assessed....
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| Published in: | Scientific reports 2021-05, Vol.11 (1), p.9582-9582, Article 9582 |
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| creator | Azimi, Akram Rezaei, Farhad Yaseri, Mehdi Jafari, Sirus Rahbar, Mohammad Douraghi, Masoumeh |
| description | Stenotrophomonas maltophilia
exhibits wide spectrum of fluoroquinolone resistance using different mechanisms as multidrug efflux pumps and Sm
qnr
alleles. Here, the role of
smeDEF
,
smeVWX
efflux genes and contribution of Sm
qnr
alleles in the development of fluoroquinolone resistance was assessed. Ciprofloxacin, levofloxacin and moxifloxacin resistance were found in 10.9%, 3.5%, and 1.6% of isolates, respectively. More than four-fold differences in ciprofloxacin MICs were detected in the presence of reserpine and
smeD, F, V
expression was significantly associated with ciprofloxacin resistance (p = 0.017 for
smeD
, 0.003 for
smeF
, and 0.001 for
smeV
). Sm
qnr
gene was found in 52% of the ciprofloxacin-resistant isolates and Sm
qnr8
was the most common allele detected. Fluoroquinolone resistance in
S. maltophilia
clinical isolates was significantly associated with active efflux pumps. There was no correlation between the Sm
qnr
alleles and ciprofloxacin resistance; however, contribution of the Sm
qnr
genes in low-level levofloxacin resistance was revealed. |
| doi_str_mv | 10.1038/s41598-021-88977-z |
| format | article |
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exhibits wide spectrum of fluoroquinolone resistance using different mechanisms as multidrug efflux pumps and Sm
qnr
alleles. Here, the role of
smeDEF
,
smeVWX
efflux genes and contribution of Sm
qnr
alleles in the development of fluoroquinolone resistance was assessed. Ciprofloxacin, levofloxacin and moxifloxacin resistance were found in 10.9%, 3.5%, and 1.6% of isolates, respectively. More than four-fold differences in ciprofloxacin MICs were detected in the presence of reserpine and
smeD, F, V
expression was significantly associated with ciprofloxacin resistance (p = 0.017 for
smeD
, 0.003 for
smeF
, and 0.001 for
smeV
). Sm
qnr
gene was found in 52% of the ciprofloxacin-resistant isolates and Sm
qnr8
was the most common allele detected. Fluoroquinolone resistance in
S. maltophilia
clinical isolates was significantly associated with active efflux pumps. There was no correlation between the Sm
qnr
alleles and ciprofloxacin resistance; however, contribution of the Sm
qnr
genes in low-level levofloxacin resistance was revealed.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-021-88977-z</identifier><identifier>PMID: 33953262</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/326/22 ; 631/326/325 ; Alleles ; Antibiotics ; Ciprofloxacin ; Clinical isolates ; Drug resistance ; Gene expression ; Humanities and Social Sciences ; Levofloxacin ; Moxifloxacin ; multidisciplinary ; Mutation ; Plasmids ; Public health ; Pumps ; Reserpine ; Science ; Science (multidisciplinary) ; Stenotrophomonas maltophilia</subject><ispartof>Scientific reports, 2021-05, Vol.11 (1), p.9582-9582, Article 9582</ispartof><rights>The Author(s) 2021</rights><rights>The Author(s) 2021. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c540t-da1f1b976bf1b4e411e9da8dcf64d782b44300e20890a66c01b66733a004f9f93</citedby><cites>FETCH-LOGICAL-c540t-da1f1b976bf1b4e411e9da8dcf64d782b44300e20890a66c01b66733a004f9f93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2522236802/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2522236802?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33953262$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Azimi, Akram</creatorcontrib><creatorcontrib>Rezaei, Farhad</creatorcontrib><creatorcontrib>Yaseri, Mehdi</creatorcontrib><creatorcontrib>Jafari, Sirus</creatorcontrib><creatorcontrib>Rahbar, Mohammad</creatorcontrib><creatorcontrib>Douraghi, Masoumeh</creatorcontrib><title>Emergence of fluoroquinolone resistance and possible mechanisms in clinical isolates of Stenotrophomonas maltophilia from Iran</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Stenotrophomonas maltophilia
exhibits wide spectrum of fluoroquinolone resistance using different mechanisms as multidrug efflux pumps and Sm
qnr
alleles. Here, the role of
smeDEF
,
smeVWX
efflux genes and contribution of Sm
qnr
alleles in the development of fluoroquinolone resistance was assessed. Ciprofloxacin, levofloxacin and moxifloxacin resistance were found in 10.9%, 3.5%, and 1.6% of isolates, respectively. More than four-fold differences in ciprofloxacin MICs were detected in the presence of reserpine and
smeD, F, V
expression was significantly associated with ciprofloxacin resistance (p = 0.017 for
smeD
, 0.003 for
smeF
, and 0.001 for
smeV
). Sm
qnr
gene was found in 52% of the ciprofloxacin-resistant isolates and Sm
qnr8
was the most common allele detected. Fluoroquinolone resistance in
S. maltophilia
clinical isolates was significantly associated with active efflux pumps. There was no correlation between the Sm
qnr
alleles and ciprofloxacin resistance; however, contribution of the Sm
qnr
genes in low-level levofloxacin resistance was revealed.</description><subject>631/326/22</subject><subject>631/326/325</subject><subject>Alleles</subject><subject>Antibiotics</subject><subject>Ciprofloxacin</subject><subject>Clinical isolates</subject><subject>Drug resistance</subject><subject>Gene expression</subject><subject>Humanities and Social Sciences</subject><subject>Levofloxacin</subject><subject>Moxifloxacin</subject><subject>multidisciplinary</subject><subject>Mutation</subject><subject>Plasmids</subject><subject>Public health</subject><subject>Pumps</subject><subject>Reserpine</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Stenotrophomonas maltophilia</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNp9kk2PFCEQhjtG427W_QMeDIkXL6189QcXE7NZdZJNPKhnUk0XM0xoGKHHZPfgb5eZ3k8Pcimg3nqA4q2q14y-Z1T0H7Jkjeprylnd96rr6ptn1Smnsqm54Pz5o_lJdZ7zlpbRcCWZelmdCKEawVt-Wv25nDCtMRgk0RLr9zHFX3sXoo8BScLs8gyHLISR7GLObvBIJjQbCC5PmbhAjHfBGfDE5ehhxnxAfZ8xxDnF3SZOMUAmE_i5rJx3QGyKE1klCK-qFxZ8xvPbeFb9_Hz54-JrffXty-ri01VtGknnegRm2aC6dihBomQM1Qj9aGwrx67ng5SCUuS0VxTa1lA2tG0nBFAqrbJKnFWrhTtG2OpdchOkax3B6eNGTGsNaXbGowYzDJ00gik7ytJY1SrVSAWcm4FLsIX1cWHt9sOEo8EwJ_BPoE8zwW30Ov7WPaOUsb4A3t0CDr3GPOvJZYPeQ8C4z5o3vPwNVbIp0rf_SLdxn0Jp1VHFRdtTXlR8UZlUfiihvb8Mo_rgFr24RRe36KNb9E0pevP4Gfcld94oArEIckmFNaaHs_-D_QsL0848</recordid><startdate>20210505</startdate><enddate>20210505</enddate><creator>Azimi, Akram</creator><creator>Rezaei, Farhad</creator><creator>Yaseri, Mehdi</creator><creator>Jafari, Sirus</creator><creator>Rahbar, Mohammad</creator><creator>Douraghi, Masoumeh</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><general>Nature Portfolio</general><scope>C6C</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20210505</creationdate><title>Emergence of fluoroquinolone resistance and possible mechanisms in clinical isolates of Stenotrophomonas maltophilia from Iran</title><author>Azimi, Akram ; 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exhibits wide spectrum of fluoroquinolone resistance using different mechanisms as multidrug efflux pumps and Sm
qnr
alleles. Here, the role of
smeDEF
,
smeVWX
efflux genes and contribution of Sm
qnr
alleles in the development of fluoroquinolone resistance was assessed. Ciprofloxacin, levofloxacin and moxifloxacin resistance were found in 10.9%, 3.5%, and 1.6% of isolates, respectively. More than four-fold differences in ciprofloxacin MICs were detected in the presence of reserpine and
smeD, F, V
expression was significantly associated with ciprofloxacin resistance (p = 0.017 for
smeD
, 0.003 for
smeF
, and 0.001 for
smeV
). Sm
qnr
gene was found in 52% of the ciprofloxacin-resistant isolates and Sm
qnr8
was the most common allele detected. Fluoroquinolone resistance in
S. maltophilia
clinical isolates was significantly associated with active efflux pumps. There was no correlation between the Sm
qnr
alleles and ciprofloxacin resistance; however, contribution of the Sm
qnr
genes in low-level levofloxacin resistance was revealed.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>33953262</pmid><doi>10.1038/s41598-021-88977-z</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| language | eng |
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| subjects | 631/326/22 631/326/325 Alleles Antibiotics Ciprofloxacin Clinical isolates Drug resistance Gene expression Humanities and Social Sciences Levofloxacin Moxifloxacin multidisciplinary Mutation Plasmids Public health Pumps Reserpine Science Science (multidisciplinary) Stenotrophomonas maltophilia |
| title | Emergence of fluoroquinolone resistance and possible mechanisms in clinical isolates of Stenotrophomonas maltophilia from Iran |
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