Angiostatin prevents IL-1β-induced down-regulation of eNOS expression by inhibiting the NF-κB cascade

• Published in: Journal of pharmacological sciences 2015-11, Vol.129 (3), p.200-204
• Main Authors: Kawasaki, Yasushi, Yokobayashi, Eriko, Sakamoto, Kentaro, Tenma, Eri, Takaki, Haruka, Chiba, Yuki, Otashiro, Tomomi, Ishihara, Mai, Yonezawa, Sei, Sugiyama, Akinori, Natori, Yasuhiro
• Format: Article
• Language: English
• Subjects: Active Transport, Cell Nucleus - drug effects; Angiostatin; Angiostatins - pharmacology; Anti-inflammatory; Cells, Cultured; Depression, Chemical; Down-Regulation - drug effects; Endothelial nitric oxide synthase (eNOS); Human Umbilical Vein Endothelial Cells - metabolism; Humans; Interleukin-1beta - adverse effects; NF-kappa B - metabolism; Nitric Oxide Synthase Type III - metabolism; Signal Transduction
• ISSN: 1347-8613; 1347-8648
• DOI: 10.1016/j.jphs.2015.10.001

This study aimed to elucidate the protective potential of angiostatin in inflamed endothelial cells in culture. We assessed the effect of angiostatin on the expression of ICAM-1 and eNOS. Angiostatin prevented IL-1β-induced down-regulation of eNOS expression, but produced no significant changes on IL-1β-induced up-regulation of ICAM-1. We then explored the effect of angiostatin on IL-1β-mediated inflammatory signaling and found that angiostatin inhibited IL-1β-mediated nuclear translocation of NF-κB. Thus, our results suggest that angiostatin prevents IL-1β-induced down-regulation of eNOS expression via inhibition of the NF-κB cascade; this may be the anti-inflammatory mechanism of angiostatin.