Intravesicular Genomic DNA Enriched by Size Exclusion Chromatography Can Enhance Lung Cancer Oncogene Mutation Detection Sensitivity

Extracellular vesicles (EVs) are cell-derived structures surrounded by a lipid bilayer that carry RNA and DNA as potential templates for molecular diagnostics, e.g., in cancer genotyping. While it has been established that DNA templates appear on the outside of EVs, no consensus exists on which nucl...

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Bibliographic Details
Published in:International journal of molecular sciences 2022-12, Vol.23 (24), p.16052
Main Authors: Van Hoof, Rebekka, Deville, Sarah, Hollanders, Karen, Berckmans, Pascale, Wagner, Patrick, Hooyberghs, Jef, Nelissen, Inge
Format: Article
Language:English
Subjects:
Acids
Biopsy
Blood plasma
Carcinoma, Non-Small-Cell Lung - diagnosis
Cell-Free Nucleic Acids
Chromatography
Chromatography, Gel
Circulating Tumor DNA
Deoxyribonucleic acid
DNA
EGFR T790M
Epidermal growth factor
Epidermal Growth Factor - genetics
Epidermal growth factor receptors
ErbB Receptors - genetics
Extracellular vesicles
Flow cytometry
Gene frequency
Genes
Genomics
Genotyping
Growth factors
Humans
intravesicular DNA
Lipid bilayers
Lipids
Lung cancer
Lung Neoplasms - diagnosis
Medical laboratories
Methods
Morphology
Mutation
Nanoparticles
non-small cell lung cancer
Non-small cell lung carcinoma
Nucleic acids
Oncogenes
Point mutation
Protein Kinase Inhibitors
Proteins
Purification
Sensitivity
Separation
Size exclusion chromatography
Tumors
Ultracentrifugation
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Description
Summary:Extracellular vesicles (EVs) are cell-derived structures surrounded by a lipid bilayer that carry RNA and DNA as potential templates for molecular diagnostics, e.g., in cancer genotyping. While it has been established that DNA templates appear on the outside of EVs, no consensus exists on which nucleic acid species inside small EVs (
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms232416052