Cloning and prokaryotic expression of WRKY48 from Caragana intermedia

( ) is a kind of drought-tolerant leguminous shrub. WRKY transcription factors are one of the largest family of transcription factors in plants and play critical regulatory roles in stress tolerance and the development of plants. In our study, was cloned from , analyzed using bioinformatics software...

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Bibliographic Details
Published in:Open life sciences 2022-03, Vol.17 (1), p.131-138
Main Authors: Liu, Jinhua, Wang, Ruigang, Li, Guojing, Wan, Yongqing
Format: Article
Language:English
Subjects:
bioinformatics analysis
caragana intermedia
CiWRKY48
prokaryotic expression system
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Summary:( ) is a kind of drought-tolerant leguminous shrub. WRKY transcription factors are one of the largest family of transcription factors in plants and play critical regulatory roles in stress tolerance and the development of plants. In our study, was cloned from , analyzed using bioinformatics software, and expressed with a prokaryotic expression system. The results showed that the open reading frame (ORF) of was 1158bp, the molecular weight (MW) was 42 kDa, and its subcellular localization was in the nucleus. Additionally, fusion protein was obtained, and confirmed by western blotting. The stress resistance of the pET30a-His-MBP-CiWRKY48 transformed expression strain was reduced under mannitol and salt treatment, compared with the control. Overall, our findings provided a foundation for uncovering the function of CiWRKY48.
ISSN:2391-5412
2391-5412
DOI:10.1515/biol-2022-0016