The role of progesterone in the regulation of miR-30a and miR-143 in rat models of subarachnoid hemorrhage: a study of dose-related effects

[LANGUAGE= "English"] INTRODUCTION: Brain damage in the early-period after subarachnoid hemorrhage (SAH) is a significant cause of mortality; however, cell proliferation, apoptosis, and inflammatory cascades play roles in preventing. This study aimed to determine the effect of progesterone...

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Published in:Boğaziçi tıp dergisi 2023-01, Vol.10 (1), p.19-25
Main Author: Ari, Buse Çağla
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acute brain injury
MicroRNAs
mir-143
mir-30a
neuroprotection
progesterone
subarachnoid hemorrhage
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description [LANGUAGE= "English"] INTRODUCTION: Brain damage in the early-period after subarachnoid hemorrhage (SAH) is a significant cause of mortality; however, cell proliferation, apoptosis, and inflammatory cascades play roles in preventing. This study aimed to determine the effect of progesterone on miR-30a and miR-143 expressions after SAH.METHODS: The research was conducted on 40 Sprague-Dawley rats. They were assigned to four groups: Group 1 with SAH (n=10); Group 2, SAH+8mg progesterone (n=10); Group 3, SAH+10mg progesterone (n=10); and Group 4, the controls (n=10). Experimental SAH was created by cisternal injection model. RNAs were isolated from brain tissues and miRNA expression levels were determined by Quantitative Real-Time PCR.RESULTS: There was a 176-fold and 126-fold decrease in miR-30a and miR-143 expressions of male rats with SAH (p
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This study aimed to determine the effect of progesterone on miR-30a and miR-143 expressions after SAH.METHODS: The research was conducted on 40 Sprague-Dawley rats. They were assigned to four groups: Group 1 with SAH (n=10); Group 2, SAH+8mg progesterone (n=10); Group 3, SAH+10mg progesterone (n=10); and Group 4, the controls (n=10). Experimental SAH was created by cisternal injection model. RNAs were isolated from brain tissues and miRNA expression levels were determined by Quantitative Real-Time PCR.RESULTS: There was a 176-fold and 126-fold decrease in miR-30a and miR-143 expressions of male rats with SAH (p<0.05). In the male rats administered with 8 mg and 16 mg progesterone, 39-fold and 2.4-fold decreases were found in miR-30a expressions (p<0.05). In the expression levels, there were a 2.3-fold decrease and a 15-fold increase in miR-30a and miR-143 in females (p<0.05). In the female rats administered with 8 mg and 16 mg progesterone, a 1.25-fold increase and 4.5-fold decrease were found in miR-30a expressions (p<0.05); however, 1400-fold and 400-fold increases were found in miR-143 expressions (p<0.05) on the females with 8 mg and 16 mg progesterone.DISCUSSION AND CONCLUSION: The decrease in miR-30a and miR-143 expressions in the males with SAH approached the normal limits by increasing doses of progesterone. We think that the molecular basis of this dose-dependent effect of progesterone in miR-30a and miR-143 expressions should be investigated in further studies.[LANGUAGE= "Turkish"] GİRİŞ ve AMAÇ: Subaraknoid kanama (SAK) sonrası erken dönemde beyin hasarı önemli bir mortalite nedenidir ancak hücre proliferasyonu, apopitoz, inflamatuvar kaskadların önlenmesinde rol oynar. Bu çalışmada, SAK sonrası miR-30a ve miR-143 ekspresyonu üzerine progesteronun etkisinin belirlenmesi amaçlandı.YÖNTEM ve GEREÇLER: Araştırma, 40 Sprague-Dawley sıçanı üzerinde gerçekleştirildi. Dört gruba ayrıldılar: SAK’lı Grup 1 (n=10); Grup 2, SAK+8 mg progesteron (n=10); Grup 3, SAK+10 mg progesteron (n=10); ve Grup 4, kontroller (n=10). Deneysel SAK, sarnıç enjeksiyon modeli ile oluşturuldu. RNA'lar beyin dokularından izole edildi ve miRNA ekspresyon seviyeleri Quantitative real-time polimeraz zincir reaksiyonu ile belirlendi.BULGULAR: SAK'lı erkek sıçanların miR-30a ve miR-143 ekspresyonlarında 176 kat ve 126 kat azalma oldu (p<0,05). 8 mg ve 16 mg progesteron uygulanan erkek sıçanlarda miR-30a ekspresyonlarında 39 kat ve 2,4 kat azalma tespit edildi (p<0,05). Ekspresyon düzeylerinde kadınlarda miR-30a ve miR-143'te 2,3 kat azalma ve 15 kat artış vardı (p<0,05). 8 mg ve 16 mg progesteron verilen dişi sıçanlarda miR-30a ekspresyonlarında 1,25 kat artış ve 4,5 kat azalma bulundu (p<0,05); ancak 8 mg ve 16 mg progesteron ile kadınlarda miR-143 ekspresyonlarında 1.400 kat ve 400 kat artışlar (p<0,05) bulundu.TARTIŞMA ve SONUÇ: SAK'lı erkeklerde miR-30a ve miR-143 ekspresyonlarındaki azalma, progesteron dozlarının artmasıyla normal sınırlara yaklaşmıştır. Progesteronun miR-30a ve miR-143 ekspresyonlarındaki bu doza bağımlı etkisinin moleküler temelinin ileri çalışmalarda araştırılması gerektiğini düşünmekteyiz.]]></description><identifier>ISSN: 2149-0287</identifier><identifier>EISSN: 2149-0287</identifier><identifier>DOI: 10.14744/bmj.2022.87049</identifier><language>eng</language><publisher>Istanbul: Kare Publishing</publisher><subject>acute brain injury ; MicroRNAs ; mir-143 ; mir-30a ; neuroprotection ; progesterone ; subarachnoid hemorrhage</subject><ispartof>Boğaziçi tıp dergisi, 2023-01, Vol.10 (1), p.19-25</ispartof><rights>2023. This work is published under https://creativecommons.org/licenses/by-nc/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/2882336672?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,25753,27924,27925,37012,44590</link.rule.ids></links><search><creatorcontrib>Ari, Buse Çağla</creatorcontrib><title>The role of progesterone in the regulation of miR-30a and miR-143 in rat models of subarachnoid hemorrhage: a study of dose-related effects</title><title>Boğaziçi tıp dergisi</title><description><![CDATA[[LANGUAGE= "English"] INTRODUCTION: Brain damage in the early-period after subarachnoid hemorrhage (SAH) is a significant cause of mortality; however, cell proliferation, apoptosis, and inflammatory cascades play roles in preventing. This study aimed to determine the effect of progesterone on miR-30a and miR-143 expressions after SAH.METHODS: The research was conducted on 40 Sprague-Dawley rats. They were assigned to four groups: Group 1 with SAH (n=10); Group 2, SAH+8mg progesterone (n=10); Group 3, SAH+10mg progesterone (n=10); and Group 4, the controls (n=10). Experimental SAH was created by cisternal injection model. RNAs were isolated from brain tissues and miRNA expression levels were determined by Quantitative Real-Time PCR.RESULTS: There was a 176-fold and 126-fold decrease in miR-30a and miR-143 expressions of male rats with SAH (p<0.05). In the male rats administered with 8 mg and 16 mg progesterone, 39-fold and 2.4-fold decreases were found in miR-30a expressions (p<0.05). In the expression levels, there were a 2.3-fold decrease and a 15-fold increase in miR-30a and miR-143 in females (p<0.05). In the female rats administered with 8 mg and 16 mg progesterone, a 1.25-fold increase and 4.5-fold decrease were found in miR-30a expressions (p<0.05); however, 1400-fold and 400-fold increases were found in miR-143 expressions (p<0.05) on the females with 8 mg and 16 mg progesterone.DISCUSSION AND CONCLUSION: The decrease in miR-30a and miR-143 expressions in the males with SAH approached the normal limits by increasing doses of progesterone. We think that the molecular basis of this dose-dependent effect of progesterone in miR-30a and miR-143 expressions should be investigated in further studies.[LANGUAGE= "Turkish"] GİRİŞ ve AMAÇ: Subaraknoid kanama (SAK) sonrası erken dönemde beyin hasarı önemli bir mortalite nedenidir ancak hücre proliferasyonu, apopitoz, inflamatuvar kaskadların önlenmesinde rol oynar. Bu çalışmada, SAK sonrası miR-30a ve miR-143 ekspresyonu üzerine progesteronun etkisinin belirlenmesi amaçlandı.YÖNTEM ve GEREÇLER: Araştırma, 40 Sprague-Dawley sıçanı üzerinde gerçekleştirildi. Dört gruba ayrıldılar: SAK’lı Grup 1 (n=10); Grup 2, SAK+8 mg progesteron (n=10); Grup 3, SAK+10 mg progesteron (n=10); ve Grup 4, kontroller (n=10). Deneysel SAK, sarnıç enjeksiyon modeli ile oluşturuldu. RNA'lar beyin dokularından izole edildi ve miRNA ekspresyon seviyeleri Quantitative real-time polimeraz zincir reaksiyonu ile belirlendi.BULGULAR: SAK'lı erkek sıçanların miR-30a ve miR-143 ekspresyonlarında 176 kat ve 126 kat azalma oldu (p<0,05). 8 mg ve 16 mg progesteron uygulanan erkek sıçanlarda miR-30a ekspresyonlarında 39 kat ve 2,4 kat azalma tespit edildi (p<0,05). Ekspresyon düzeylerinde kadınlarda miR-30a ve miR-143'te 2,3 kat azalma ve 15 kat artış vardı (p<0,05). 8 mg ve 16 mg progesteron verilen dişi sıçanlarda miR-30a ekspresyonlarında 1,25 kat artış ve 4,5 kat azalma bulundu (p<0,05); ancak 8 mg ve 16 mg progesteron ile kadınlarda miR-143 ekspresyonlarında 1.400 kat ve 400 kat artışlar (p<0,05) bulundu.TARTIŞMA ve SONUÇ: SAK'lı erkeklerde miR-30a ve miR-143 ekspresyonlarındaki azalma, progesteron dozlarının artmasıyla normal sınırlara yaklaşmıştır. Progesteronun miR-30a ve miR-143 ekspresyonlarındaki bu doza bağımlı etkisinin moleküler temelinin ileri çalışmalarda araştırılması gerektiğini düşünmekteyiz.]]></description><subject>acute brain injury</subject><subject>MicroRNAs</subject><subject>mir-143</subject><subject>mir-30a</subject><subject>neuroprotection</subject><subject>progesterone</subject><subject>subarachnoid hemorrhage</subject><issn>2149-0287</issn><issn>2149-0287</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNpNkU9r3DAQxU1ooSHJuVdBzt7oz8iSegshbQOBQkjPYiyNd73YVirZh3yGfunau6X0NI-ZH2-GeVX1WfCdAANw147HneRS7qzh4C6qSynA1Vxa8-E__am6KeXI-aqdddpdVr9fD8RyGoiljr3ltKcyU04TsX5i8zaj_TLg3KdpI8b-pVYcGU7xpAWoDcw4szFFGsoGlaXFjOEwpT6yA40p5wPu6QtDVuYlvm9MTIXqTKszRUZdR2Eu19XHDodCN3_rVfXz6-Prw_f6-ce3p4f75zoIo13tgqMuWNIarQETUMfYNhRM0OBEi0qBjoIrCLxtWm5aQGoMhEYZFJqCuqqezr4x4dG_5X7E_O4T9v7USHnvMc99GMhj1I4cBKMEB-1sa7EBaAEcB2kbu3rdnr3W3_1a1uf5Y1rytJ7vpbVSqaYxcqXuzlTIqZRM3b-tgvtTgH4N0G8B-lOA6g8hxY1J</recordid><startdate>20230101</startdate><enddate>20230101</enddate><creator>Ari, Buse Çağla</creator><general>Kare Publishing</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>DOA</scope></search><sort><creationdate>20230101</creationdate><title>The role of progesterone in the regulation of miR-30a and miR-143 in rat models of subarachnoid hemorrhage: a study of dose-related effects</title><author>Ari, Buse Çağla</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1759-9c9efc8e55a8747ca5ddb6ec7c5491ba3345d1034c0b6b07b4ae674c637a15ec3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>acute brain injury</topic><topic>MicroRNAs</topic><topic>mir-143</topic><topic>mir-30a</topic><topic>neuroprotection</topic><topic>progesterone</topic><topic>subarachnoid hemorrhage</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ari, Buse Çağla</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Directory of Open Access Journals</collection><jtitle>Boğaziçi tıp dergisi</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ari, Buse Çağla</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The role of progesterone in the regulation of miR-30a and miR-143 in rat models of subarachnoid hemorrhage: a study of dose-related effects</atitle><jtitle>Boğaziçi tıp dergisi</jtitle><date>2023-01-01</date><risdate>2023</risdate><volume>10</volume><issue>1</issue><spage>19</spage><epage>25</epage><pages>19-25</pages><issn>2149-0287</issn><eissn>2149-0287</eissn><abstract><![CDATA[[LANGUAGE= "English"] INTRODUCTION: Brain damage in the early-period after subarachnoid hemorrhage (SAH) is a significant cause of mortality; however, cell proliferation, apoptosis, and inflammatory cascades play roles in preventing. This study aimed to determine the effect of progesterone on miR-30a and miR-143 expressions after SAH.METHODS: The research was conducted on 40 Sprague-Dawley rats. They were assigned to four groups: Group 1 with SAH (n=10); Group 2, SAH+8mg progesterone (n=10); Group 3, SAH+10mg progesterone (n=10); and Group 4, the controls (n=10). Experimental SAH was created by cisternal injection model. RNAs were isolated from brain tissues and miRNA expression levels were determined by Quantitative Real-Time PCR.RESULTS: There was a 176-fold and 126-fold decrease in miR-30a and miR-143 expressions of male rats with SAH (p<0.05). In the male rats administered with 8 mg and 16 mg progesterone, 39-fold and 2.4-fold decreases were found in miR-30a expressions (p<0.05). In the expression levels, there were a 2.3-fold decrease and a 15-fold increase in miR-30a and miR-143 in females (p<0.05). In the female rats administered with 8 mg and 16 mg progesterone, a 1.25-fold increase and 4.5-fold decrease were found in miR-30a expressions (p<0.05); however, 1400-fold and 400-fold increases were found in miR-143 expressions (p<0.05) on the females with 8 mg and 16 mg progesterone.DISCUSSION AND CONCLUSION: The decrease in miR-30a and miR-143 expressions in the males with SAH approached the normal limits by increasing doses of progesterone. We think that the molecular basis of this dose-dependent effect of progesterone in miR-30a and miR-143 expressions should be investigated in further studies.[LANGUAGE= "Turkish"] GİRİŞ ve AMAÇ: Subaraknoid kanama (SAK) sonrası erken dönemde beyin hasarı önemli bir mortalite nedenidir ancak hücre proliferasyonu, apopitoz, inflamatuvar kaskadların önlenmesinde rol oynar. Bu çalışmada, SAK sonrası miR-30a ve miR-143 ekspresyonu üzerine progesteronun etkisinin belirlenmesi amaçlandı.YÖNTEM ve GEREÇLER: Araştırma, 40 Sprague-Dawley sıçanı üzerinde gerçekleştirildi. Dört gruba ayrıldılar: SAK’lı Grup 1 (n=10); Grup 2, SAK+8 mg progesteron (n=10); Grup 3, SAK+10 mg progesteron (n=10); ve Grup 4, kontroller (n=10). Deneysel SAK, sarnıç enjeksiyon modeli ile oluşturuldu. RNA'lar beyin dokularından izole edildi ve miRNA ekspresyon seviyeleri Quantitative real-time polimeraz zincir reaksiyonu ile belirlendi.BULGULAR: SAK'lı erkek sıçanların miR-30a ve miR-143 ekspresyonlarında 176 kat ve 126 kat azalma oldu (p<0,05). 8 mg ve 16 mg progesteron uygulanan erkek sıçanlarda miR-30a ekspresyonlarında 39 kat ve 2,4 kat azalma tespit edildi (p<0,05). Ekspresyon düzeylerinde kadınlarda miR-30a ve miR-143'te 2,3 kat azalma ve 15 kat artış vardı (p<0,05). 8 mg ve 16 mg progesteron verilen dişi sıçanlarda miR-30a ekspresyonlarında 1,25 kat artış ve 4,5 kat azalma bulundu (p<0,05); ancak 8 mg ve 16 mg progesteron ile kadınlarda miR-143 ekspresyonlarında 1.400 kat ve 400 kat artışlar (p<0,05) bulundu.TARTIŞMA ve SONUÇ: SAK'lı erkeklerde miR-30a ve miR-143 ekspresyonlarındaki azalma, progesteron dozlarının artmasıyla normal sınırlara yaklaşmıştır. Progesteronun miR-30a ve miR-143 ekspresyonlarındaki bu doza bağımlı etkisinin moleküler temelinin ileri çalışmalarda araştırılması gerektiğini düşünmekteyiz.]]></abstract><cop>Istanbul</cop><pub>Kare Publishing</pub><doi>10.14744/bmj.2022.87049</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record>
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subjects acute brain injury
MicroRNAs
mir-143
mir-30a
neuroprotection
progesterone
subarachnoid hemorrhage
title The role of progesterone in the regulation of miR-30a and miR-143 in rat models of subarachnoid hemorrhage: a study of dose-related effects
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