Bioproduction of Quercetin and Rutinose Catalyzed by Rutinosidase: Novel Concept of "Solid State Biocatalysis"

• Published in: International journal of molecular sciences 2019-03, Vol.20 (5), p.1112
• Main Authors: Kapešová, Jana, Petrásková, Lucie, Markošová, Kristína, Rebroš, Martin, Kotik, Michael, Bojarová, Pavla, Křen, Vladimír
• Format: Article
• Language: English
• Subjects: Antioxidants; Aspergillus niger; Aspergillus niger - enzymology; Aspergillus niger - genetics; Biocatalysis; Bioconversion; Biomass; Catalysis; Denaturation; Disaccharides - chemistry; Disaccharides - metabolism; Enzymes; Evaporation; Fermentation; Flammability; Flavonoids; Fruits; Fungal Proteins - genetics; Fungal Proteins - metabolism; Glycoside Hydrolases - genetics; Glycoside Hydrolases - metabolism; Industrial Microbiology - methods; Methods; Pichia - genetics; Pichia - metabolism; Quercetin; Quercetin - chemistry; Quercetin - metabolism; Rutin; Rutin - chemistry; Rutin - metabolism; rutinose; rutinosidase; solid-state biocatalysis; Solubility
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms20051112

Quercetin is a flavonoid largely employed as a phytochemical remedy and a food or dietary supplement. We present here a novel biocatalytic methodology for the preparation of quercetin from plant-derived rutin, with both substrate and product being in mostly an undissolved state during biotransformation. This "solid-state" enzymatic conversion uses a crude enzyme preparation of recombinant rutinosidase from yielding quercetin, which precipitates from virtually insoluble rutin. The process is easily scalable and exhibits an extremely high space-time yield. The procedure has been shown to be robust and was successfully tested with rutin concentrations of up to 300 g/L (ca 0.5 M) at various scales. Using this procedure, pure quercetin is easily obtained by mere filtration of the reaction mixture, followed by washing and drying of the filter cake. Neither co-solvents nor toxic chemicals are used, thus the process can be considered environmentally friendly and the product of "bio-quality." Moreover, rare disaccharide rutinose is obtained from the filtrate at a preparatory scale as a valuable side product. These results demonstrate for the first time the efficiency of the "Solid-State-Catalysis" concept, which is applicable virtually for any biotransformation involving substrates and products of low water solubility.