Glucose Sensor Using Sol–Gel Coating Layer Deposited on PMMA Optical Fiber: An Enzyme Activity Measurement System

In the present work, a biocatalytic glucose optical sensor produced by immobilizing glucose oxidase (GOD) as a recognition molecule over a PMMA (polymethylmethacrylate) optical fiber is introduced. An enzymatic encapsulation process was carried out using the sol–gel method, depositing a TEOS-based c...

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Bibliographic Details
Published in:Gels 2023-07, Vol.9 (8), p.608
Main Authors: Ortega-Contreras, Jorge-A, Alvarado-Méndez, Edgar, Almanza-Rodríguez, Guillermo, Hernández, María del Carmen, Celaya-García, Luis
Format: Article
Language:English
Subjects:
biosensor
Biosensors
Book publishing
Coating
Detectors
Dextrose
Encapsulation
Enzyme activity
Enzymes
Equipment and supplies
Fiber optics
Glucose
Glucose oxidase
Grain size
Measuring instruments
Optical fibers
Optical measuring instruments
Optics
Oxidases
Oxidation
Polymethyl methacrylate
Polymethylmethacrylate
Room temperature
Sensors
Sol-gel processes
sol–gel
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Summary:In the present work, a biocatalytic glucose optical sensor produced by immobilizing glucose oxidase (GOD) as a recognition molecule over a PMMA (polymethylmethacrylate) optical fiber is introduced. An enzymatic encapsulation process was carried out using the sol–gel method, depositing a TEOS-based coating by immersion at the end of an optical fiber; the biosensor was characterized using different glucose levels. Finally, the best way to encapsulate the enzyme and prevent it from degrading is to perform the process at room temperature, and later implement the deposition of the coating on the fiber. The drying process was optimal below 8 °C.
ISSN:2310-2861
2310-2861
DOI:10.3390/gels9080608