The application of metagenomic next-generation sequencing in diagnosing Chlamydia psittaci pneumonia: a report of five cases

Chlamydia psittaci pneumonia is a zoonotic infectious disease caused by Chlamydia psittaci. Diagnostic tools, including culture, serologic test and PCR-based methods, are available but prone to false negative results. This report included five cases of Chlamydia psittaci pneumonia. Symptoms and sign...

Full description

Saved in:
Bibliographic Details
Published in:BMC pulmonary medicine 2020-03, Vol.20 (1), p.65-65, Article 65
Main Authors: Gu, Lei, Liu, Wei, Ru, Meng, Lin, Jing, Yu, Guoqing, Ye, Jia, Zhu, Zheng-An, Liu, Yuebin, Chen, Jian, Lai, Guoxiang, Wen, Wen
Format: Article
Language:English
Subjects:
Aged
Aged, 80 and over
Birds
Body temperature
Bronchoalveolar Lavage Fluid - microbiology
Cardiovascular disease
Case Report
Case reports
CAT scans
Causes of
Chlamydia
Chlamydia infections
Chlamydia psittaci
Chlamydophila psittaci - genetics
Chlamydophila psittaci - isolation & purification
Communicable diseases
Diabetes
Diagnosis
Diagnostic imaging
Diseases
DNA sequencing
Doxycycline
Female
Fever
Genetic aspects
Health aspects
High-Throughput Nucleotide Sequencing
Humans
Infections
Laboratories
Lung diseases
Lungs
Lymphocytes
Male
Medical imaging
Meropenem
Metagenomics
Metronidazole
Middle Aged
mNGS
Moxifloxacin
Neutrophils
Next-generation sequencing
Parrot fever
Pathogens
Pneumonia
Pneumonia, Bacterial - diagnosis
Pneumonia, Bacterial - physiopathology
Psittacosis - diagnosis
Psittacosis - physiopathology
Pulmonology
Tomography, X-Ray Computed
Zoonoses
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Chlamydia psittaci pneumonia is a zoonotic infectious disease caused by Chlamydia psittaci. Diagnostic tools, including culture, serologic test and PCR-based methods, are available but prone to false negative results. This report included five cases of Chlamydia psittaci pneumonia. Symptoms and signs common to all 5 cases included fever, coughing, generalized muscle ache, and most notably, inflammatory infiltration of the lungs upon chest CT and X-ray. Metagenomic next-generation sequencing (mNGS) revealed the presence of Chlamydia psittaci in biopsy lung tissue in 3 cases and bronchoalveolar lavage fluid in the remaining 2 cases. Three patients responded to doxycycline plus moxifloxacin; two patients responded to moxifloxacin alone. mNGS could be used to diagnose Chlamydia psittaci pneumonia.
ISSN:1471-2466
1471-2466
DOI:10.1186/s12890-020-1098-x