Genomic Characterization of Carbapenemase-Producing Enterobacter hormaechei , Serratia marcescens , Citrobacter freundii , Providencia stuartii , and Morganella morganii Clinical Isolates from Bulgaria

Carbapenemase-producing spp. , , spp., and (CP-ESCPM) are increasingly identified as causative agents of nosocomial infections but are still not under systematic genomic surveillance. In this study, using a combination of whole-genome sequencing and conjugation experiments, we sought to elucidate th...

Full description

Saved in:
Bibliographic Details
Published in:Antibiotics (Basel) 2024-05, Vol.13 (5), p.455
Main Authors: Sabtcheva, Stefana, Stoikov, Ivan, Ivanov, Ivan N, Donchev, Deyan, Lesseva, Magdalena, Georgieva, Sylvia, Teneva, Deana, Dobreva, Elina, Christova, Iva
Format: Article
Language:English
Subjects:
Bacteria
Carbapenemase
carbapenemases
Citrobacter
Citrobacter freundii
Clinical isolates
Conjugation
Enterobacter hormaechei
Enzymes
Gene sequencing
Genes
Genomes
Genomics
horizontal gene transfer
Localization
Morganella morganii
Mutation
NDM-1
Nosocomial infection
Nosocomial infections
OXA-48
Plasmids
Providencia stuartii
Serratia marcescens
VIM-4
VIM-86
Whole genome sequencing
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Carbapenemase-producing spp. , , spp., and (CP-ESCPM) are increasingly identified as causative agents of nosocomial infections but are still not under systematic genomic surveillance. In this study, using a combination of whole-genome sequencing and conjugation experiments, we sought to elucidate the genomic characteristics and transferability of resistance genes in clinical CP-ESCPM isolates from Bulgaria. Among the 36 sequenced isolates, NDM-1 (12/36), VIM-4 (11/36), VIM-86 (8/36), and OXA-48 (7/36) carbapenemases were identified; two isolates carried both NDM-1 and VIM-86. The majority of carbapenemase genes were found on self-conjugative plasmids. IncL plasmids were responsible for the spread of OXA-48 among , , and . IncM2 plasmids were generally associated with the spread of NDM-1 in and , and also of VIM-4 in . IncC plasmids were involved in the spread of the recently described VIM-86 in isolates. IncC plasmids carrying and were observed too. was also detected on IncX3 in and on IncT plasmid in . The significant resistance transfer rates we observed highlight the role of the ESCPM group as a reservoir of resistance determinants and stress the need for strengthening infection control measures.
ISSN:2079-6382
2079-6382
DOI:10.3390/antibiotics13050455