LC-MS-based method for the qualitative and quantitative analysis of complex lipid mixtures

A simple and robust LC-MS-based methodology for the investigation of lipid mixtures is described, and its application to the analysis of human lipoprotein-associated lipids is demonstrated. After an optional initial fractionation on Silica 60, normal-phase HPLC-MS on a YMC PVA-Sil column is used fir...

Full description

Saved in:
Bibliographic Details
Published in:Journal of lipid research 2006-04, Vol.47 (4), p.804-814
Main Authors: Sommer, Ulf, Herscovitz, Haya, Welty, Francine K., Costello, Catherine E.
Format: Article
Language:English
Subjects:
Chromatography, Liquid - methods
familial hypobetalipoproteinemia
Gangliosides - analysis
Humans
intermediate density lipoprotein
Lipids - analysis
Lipoproteins - analysis
low density lipoprotein
Mass Spectrometry - methods
normal-phase high-performance liquid chromatography-mass spectrometry
reversed-phase liquid chromatography-tandem mass spectrometry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A simple and robust LC-MS-based methodology for the investigation of lipid mixtures is described, and its application to the analysis of human lipoprotein-associated lipids is demonstrated. After an optional initial fractionation on Silica 60, normal-phase HPLC-MS on a YMC PVA-Sil column is used first for class separation, followed by reversed-phase LC-MS or LC-tandem mass spectrometry using an Atlantis dC18 capillary column, and/or nanospray MS, to fully characterize the individual lipids. The methodology is applied here for the analysis of human apolipoprotein B-associated lipids. This approach allows for the determination of even low percentages of lipids of each molecular species and showed clear differences between lipids associated with apolipoprotein B-100-LDL isolated from a normal individual and those associated with a truncated version, apolipoprotein B-67-containing lipoproteins, isolated from a homozygote patient with familial hypobetalipoproteinemia. The methods described should be easily adaptable to most modern MS instrumentation.
ISSN:0022-2275
1539-7262
DOI:10.1194/jlr.M500506-JLR200