RAB2A promotes cervical cancer progression as revealed by comprehensive analysis of HPV integration and proteome in longitudinal cervical samples

• Published in: Clinical and translational medicine 2022-03, Vol.12 (3), p.e767-n/a
• Main Authors: Meng, Yifan, Lin, Shitong, Zhou, Yi, Yi, Xiao, Wu, Ping, Zhang, Qing, Ge, Weigang, Cao, Canhui, Gao, Peipei, Zhi, Wenhua, Peng, Ting, Wei, Juncheng, Ding, Wencheng, Ma, Ding, Li, Guoliang, Yang, Qin, Guo, Tiannan, Zeng, Xi, Wu, Peng
• Format: Article
• Language: English
• Subjects: Biomarkers; Cervical cancer; Cervix Uteri; Female; Genomes; Human papillomavirus; Humans; Letter to Editor; Medical prognosis; Metastasis; Papillomaviridae - genetics; Papillomavirus Infections - genetics; Proteins; Proteome; Uterine Cervical Neoplasms
• ISSN: 2001-1326; 2001-1326
• DOI: 10.1002/ctm2.767

Analysis of cervical squamous cell carcinoma shows that human papillomavirus (HPV) integration occurs in more than 80% of cervical cancers,1 suggesting the integrations of HPV genomic DNA plays an important role in promoting oncogenesis.2 In the present study, we enrolled 101 longitudinal monitoring patients (Figure S1) and collected both HPV+ normal cervical epithelium and cervical intraepithelial neoplasia (CIN) tissues to detect HPV integrations (Tables S1 and S2).3–5 A total of 1041 high-frequency HPV integrations were thus obtained (Figure S2, Table S3). SEE PDF] To evaluate the effects of HPV integrations on the protein expression during carcinogenesis, we then examined the associations between HPV integrations and proteomic data (Figure S10). Because RAB2A basal expression in the C33A and ME180 cell lines was higher than that in the SiHa and HeLa cell lines (Figure 3A and B), C33A and ME180 cells were therefore transfected with RAB2A siRNA (Figure 3C and D) and showed significantly decreased scratch wound healing and lower migration abilities (Table S8; Figures 3E–G and S17).