Cytotoxicity and genotoxicity of bioceramic root canal sealers compared to conventional resin-based sealer

The aim of this study was to evaluate cytotoxicity and genotoxicity of calcium-silicate based sealers and comparing them with a gold standard—an epoxy-based sealant. Two experimental cell lines were used, gingival fibroblasts (hGF) and monocyte/macrophage peripheral blood cell line (SC). The cytotox...

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Bibliographic Details
Published in:Scientific reports 2024-02, Vol.14 (1), p.4124-4124, Article 4124
Main Authors: Radwanski, Mateusz, Rozpedek-Kaminska, Wioletta, Galita, Grzegorz, Siwecka, Natalia, Sokolowski, Jerzy, Majsterek, Ireneusz, Özcan, Mutlu, Lukomska-Szymanska, Monika
Format: Article
Language:English
Subjects:
692/700/3032
692/700/3032/3065
692/700/3032/3065/3070
692/700/3032/3123
Apoptosis
Bioassays
Cell cycle
Cell lines
Cell viability
Comet assay
Cytotoxicity
DNA damage
Endodontics
Genotoxicity
Humanities and Social Sciences
Macrophages
Monocytes
multidisciplinary
Peripheral blood
Root canals
Science
Science (multidisciplinary)
Sealants
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Summary:The aim of this study was to evaluate cytotoxicity and genotoxicity of calcium-silicate based sealers and comparing them with a gold standard—an epoxy-based sealant. Two experimental cell lines were used, gingival fibroblasts (hGF) and monocyte/macrophage peripheral blood cell line (SC). The cytotoxicity (XTT assay) and genotoxicity (comet assay) were evaluated both after 24-h and 48-h incubation. Additionally, after 48-h incubation, the cell apoptosis and cell cycle progression was detected. BioRoot Flow induced a significant decrease in hGF cells viability compared to the negative control groups both after 24-h ( p  
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-024-54726-1